pLEXSY-sat2 Cat-No. EGE-234

Description:

nt 1-686:		5«part of 18S rRNA of Leishmania 
nt 698-1085:		5«non-translated region of aprt gene of Leishmania 
			with splice acceptor site for target gene
nt 1085-1104: 		MCS
nt 1094-1162 :		signal peptide LMSAP
nt 1154-1176 :		MCS  
nt 1177-2152:		stuffer
nt 2153-2170:		MCS
nt 2200-3538:		1.4k intergenic region from cam operon of Leishmania 
with polyA site for target gene and splice acceptor site for marker gene
nt 3549-4073:		sat marker gene
nt 4123-4696:	 5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene
nt 4697-5781:		3«part of 18S rRNA of Leishmania
nt 5786-8624:		pBluescript II KS (-) with ori and bla marker gene @ 6999-7859


> pLEXSY-sat2 Cat-No. EGE-234
AAATTGGATAACTTGGCGAAACGCCAAGCTAATACATGAACCAACCGGGTGTTCTCCACTCC
AGACGGTGGGCAACCATCGTCGTGAGACGCCCAGCGAATGAATGACAGTAAAACCAATGCC
TTCACTGGCAGTAACACCCAGCAGTGTTGACTCAATTCATTCCGTGCGAAAGCCGGCTTGTT
CCGGCGTCTTTTGACGAACAACTGCCCTATCAGCTGGTGATGGCCGTGTAGTGGACTGCCA
TGCATGGCGTTGACGGGAGCGGGGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA
TAGCTACCACTTCTACGGAGGGCAGCAGGCGCGCAAATTGCCCAATGTCAAAACAAAACGA
TGAGGCAGCGAAAAGAAATAGAGTTGTCAGTCCATTTGGATTGTCATTTCAATGGGGGATAT
TTAAACCCATCCAATATCGAGTAACAATTGGAGGACAAGTCTGGTGCCAGCACCCGCGGTAA
TTCCAGCTCCAAAAGCGTATATTAATGCTGTTGCTGTTAAAGGGTTCGTAGTTGAACTGTGG
GCTGTGCAGGTTTGTTCCTGGTCGTCCCGTCCATGTCGGATTTGGTGACCCAGGCCCTTGC
AGCCCGTGAACATTCAAAGAAACAAGAAACACGGGAGTGGTTCCTTTCCTGATTTACGCATG
TCATGCATGCGTCGAGGATCCAGTCGCAGCCTGACCGCATCACACATCAAGGCGTTACAGC
CTCTCCGTCTTTGCTGCACCCAAGTGTATTCGTGTGACCGCGTTGAGGGATCTAATGGATTC
GGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCCGTGCCTCTC
TGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGCGTGCGCCATCTGACAA
CGAATAGATCAGCAGCATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTA
TTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGC
TCTTCACGCTCCTGCTTTCCTTGCTGTGCCTTGCCACCAGATCTGCCATGGCCTCGAGGCTC
GTCCGTGTGCTGGCCGCCGCCATGCTGGTTGCAGCGGCCGTGTCGGTCGACGCTGGCGCC
TCTCTAGACACAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGT
GCTCGAGAAGAATGTGACAGTGACACACTCTGTTAACCTGCTCGAAGACAGCCACAACGGA
AAACTATGTAGATTAAAAGGAATAGCCCCACTACAATTGGGGAAATGTAACATCGCCGGATG
GCTCTTGGGAAACCCAGAATGCGACCCACTGCTTCCAGTGAGATCATGGTACTACATTGTAG
AAACACCAAACTCTGAGAATGGAATATGTTATCCAGGAGATTTCATCGACTATGAGGAGCTG
AGGGAGCAATTGAGCTCAGTGTCATCATTCGAAAGATTCGAAATATTTCCCAAAGAAAGCTC
ATGGCCCAACCACAACACAAACAAAGGAGTAACGGCAGCATGCTCCCATGCGGGGGAAAGC
AGTTTTTACAGAAATTTGCTATGGCTGACGGAGAAGGAGGGCTCATACCCAAAGCTGAAAAA
TTCTTATGTGAACAAGAGAGGGAAAGAAGTCCTTGTACTGTGGGGTATTCATCACCCGTCTA
ACAGTAAGGAGCAACAGAATATCTATCAGAATGAAAATGCTTATGTCTCTGTAGTGACTTCAA
ATTATAACAGGAGATTTACCCCGGAAATAGCAGAAAGACCCAAAGTAAGAGATCAAGCTGGG
AGGATGAACTATTACTGGACCTTGCTAAAACCCGGAGACACAATAATATTTGAGGCAAATGG
AAATCTAATAGCACCAAGGTATGCTTTCGCACTGAGTAGAGGCTTTGGGTCCGGCATCATCA
CCTCAAACGCATCAATGCATGAGTGTAACACGAAGTGTCAAACACCCCTGGGAGCTATAAAC
AGCAGTCTCCCTTTCCAGAATATACACCCAGTCACAATAGGAGAGTGCCCAAAATACGTCAG
GAGTGCCAAATTGAGGATGGTTACAGGACTAAGGAACATTCCGTCCATTCAATCCGCTAGCC
TTAAGGGTACCCACCACCATCACCACCACTAGGCGGCCGCCCTCCTCCTCCTTTCTTGTTCC
TTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGACGAGTCTTACTTTTACGTGTACTTCT
CTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATGTGTCCGTGTGTTGTGTACG
CGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCATGATGCGTGTGTATA
TCCACGCGCAGGCACGCACACACACACACACACACACACACACAGGCACACACAGGCACAC
AAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGATCATGTAG
TTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAAGCCCG
ATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGGA
GGGGGGAAGGGAGGCATAAACGGATCGATCCCCCATCGCACATGCGGGTACGAGCATTAT
CTGCTGTGTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCC
CCCCCCCGCGGCTCCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCA
TGTCACGAAGAGAAAAGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGC
ATGCAGACGTCGAGCCGTGGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAG
GAGATTTACTGCAGGACGTCTACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAG
GAAAATTGGGGGGGAGGGCGCACGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACT
CTCAACTTGTGTCCGTATTGCGAAGGAGGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAA
ACGTCGGAACCACCTATGAATCCCACTTCTGCGTCGCGGAGGCGTACTGCTTCCGACCACC
TCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTTCCCCTTCTTTCCGTTGTGTGTGTGTGTG
TGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCAACCCTCCACGCCTTCCTCTCCC
CCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTATCACATCCACCACCACTCT
TACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCCGCCTCTCTCGCTCG
CTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCCCACCCACCCACCCA
CCCACCCACCGCTCTACACACCTCCACAGCAGCCAGATCCTAGTATGAAGATTTCGGTGATC
CCTGAGCAGGTGGCGGAAACATTGGATGCTGAGAACCATTTCATTGTTCGTGAAGTGTTCGA
TGTGCACCTATCCGACCAAGGCTTTGAACTATCTACCAGAAGTGTGAGCCCCTACCGGAAG
GATTACATCTCGGATGATGACTCTGATGAAGACTCTGCTTGCTATGGCGCATTCATCGACCA
AGAGCTTGTCGGGAAGATTGAACTCAACTCAACATGGAACGATCTAGCCTCTATCGAACACA
TTGTTGTGTCGCACACGCACCGAGGCAAAGGAGTCGCGCACAGTCTCATCGAATTTGCGAA
AAAGTGGGCACTAAGCAGACAGCTCCTTGGCATACGATTAGAGACACAAACGAACAATGTAC
CTGCCTGCAATTTGTACGCAAAATGTGGCTTTACTCTCGGCGGCATTGACCTGTTCACGTAT
AAAACTAGACCTCAAGTCTCGAACGAAACAGCGATGTACTGGTACTGGTTCTCGGGAGCACA
GGATGACGCCTAACTAGCCTCGGAGATCCACTAGTTCTAGTTCTAGGGGCCGAATTCAGATC
CTCGTGTGAGCGTTCGCGGAATCGGTCGCTCGTGTTTATGCCCGTCTTGGTGTTGTGCTCG
CAAGGCGGTGCAGCAGGATACCGTCGCCCTCCTCTCTCCTTGCTTCTCTGTTCTTCAATTCG
CGATCTCACAGAGGCCGGCTGTGCACGCCCTTCCTCACCCTCCTTTTCCCACCTCTCGGCC
ACCGGTCGGCTCCGTTCCGTCTGCCGTCGAGAAGGGACGGGCATGTGCAGCTCCTCCCTTT
CTCTCGCGCGCGCATCTTCTCTTGCTTGTGGCACTCACGCTCATGCGTCAAGGCGGCCCCA
CGCCGAGCCCCTGCGCTCCCTTCCCTCTTGCGCATCCGTAGCGCGGATGCCGTCGATGCG
CAAGGCCGGCATGAAGGAGCGCGTGCCCTCAAGAGGGCACACTATCATGCCCTACGTGGG
CCACGCAGCGATGAGGCCGGCTTCGGCGGAGATGCGTCACGCACGTGCCAGATGATGCGC
TACGCCTTCCTTGACTTGCGCCCCCCTCTCTTCCTCCGTCTCTCACTCTCTCTCTCTCACACA
CACACACACACACACACACACACAAAGCTCCGGTTCGTCCGGCCGTAACGCCTTTTCAACTC
ACGGCCTCTAGGAATGAAGGAGGGTAGTTCGGGGGAGAACGTACTGGGGCGTCAGAGGTG
AAATTCTTAGACCGCACCAAGACGAACTACAGCGAAGGCATTCTTCAAGGATACCTTCCTCA
ATCAAGAACCAAAGTGTGGAGATCGAAGATGATTAGAGACCATTGTAGTCCACACTGCAAAC
GATGACACCCATGAATTGGGGATCTTATGGGCCGGCCTGCGGCAGGGTTTACCCTGTGTCA
GCACCGCGCCCGCTTTTACCAACTTACGTATCTTTTCTATTCGGCCTTTACCGGCCACCCAC
GGGAATATCCTCAGCACGTTTTCTGTTTTTTCACGCGAAAGCTTTGAGGTTACAGTCTCAGG
GGGGAGTACGTTCGCAAGAGTGAAACTTAAAGAAATTGACGGAATGGCACCACAAGACGTG
GAGCGTGCGGTTTAATTTGACTCAACACGGGGAACTTTACCAGATCCGGACAGGATGAGGA
TTGACAGATTGAGTGTTCTTTCTCGATTCCCTGAATGGTGGTGCATGGCCGCTTTTGGTCGG
TGGAGTGATTTGTTTGGTTGATTCCGTCAACGGACGAGATCCAAGCTGCCCAGTAGAATTCA
GAATTGCCCATAGAATAGCAAACTCATCGGCGGGTTTTACCCAACGGTGGGCCGCATTCGG
TCGAATTCTTCTCTGCGGGATTCCTTTGTAATTGCACAAGGTGAAATTTTGGGCAACAGCAG
GTCTGTGATGCTCCTCAATGTTCTGGGCGACACGCGCACTACAATGTCAGTGAGAACAAGAA
AAACGACTTTTGTCGAACCTACTTGATCAAAAGAGTGGGGAAACCCCGGAATCACATAGACC
CACTTGGGACCGAGGATTGCAATTATTGGTCGCGCAACGAGGAATGTCTCGTAGGCGCAGC
TCATCAAACTGTGCCGATTACGTCCCTGCCATTTGTACACACCGCCCGTCGTTGTTTCCGAT
GATGGTGCAATACAGGTGATCGGACAGGCGGTGTTTTATCCGCCCGAAAGTTCACCGATATT
TAAATCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATA
GCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCA
TAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCA
CTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCG
CGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG
CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC
ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC
AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCG
CCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACA
GAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGC
TCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCT
CAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTA
AGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGA
AGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA
GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTC
GTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGC
GAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGA
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAG
CTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATC
GTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGC
GAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT
GTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCT
TACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTG
AGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCG
CCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTC
AAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTT
CAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCA
AAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTAT
TGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATA
AACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGG
CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGC
CCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCC
GTCAAGCTCTAAATCGGGGGCGCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA
CCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTT
TTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA
CACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATT
GGTTAAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTA
CAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCT
TCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGC
CAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTC
ACTATAGGGCGAATTGGATTT