pLEXSY-neo2 Cat-No. EGE-233

Description:

nt 1-686:		5«part of 18S rRNA of Leishmania 
nt 698-1085:		5«non-translated region of aprt gene of Leishmania 
			with splice acceptor site for target gene
nt 1085-1104: 		MCS
nt 1094-1162 :		signal peptide LMSAP
nt 1154-1176 :		MCS  
nt 1177-2152:		stuffer
nt 2153-2170:		MCS
nt 2200-3538:		1.4k intergenic region from cam operon of Leishmania 
with polyA site for target gene and splice acceptor site for marker gene
nt 3548-4351:		neo marker gene
nt 4385-4958:	 5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene
nt 4959-6043:		3«part of 18S rRNA of Leishmania
nt 6048-8904:		pBluescript II KS (-) with ori and bla marker gene @ 7261-8121


> pLEXSY-neo2 Cat-No. EGE-233
AAATTGGATAACTTGGCGAAACGCCAAGCTAATACATGAACCAACCGGGTGTTCTCCACTCC
AGACGGTGGGCAACCATCGTCGTGAGACGCCCAGCGAATGAATGACAGTAAAACCAATGCC
TTCACTGGCAGTAACACCCAGCAGTGTTGACTCAATTCATTCCGTGCGAAAGCCGGCTTGTT
CCGGCGTCTTTTGACGAACAACTGCCCTATCAGCTGGTGATGGCCGTGTAGTGGACTGCCA
TGCATGGCGTTGACGGGAGCGGGGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA
TAGCTACCACTTCTACGGAGGGCAGCAGGCGCGCAAATTGCCCAATGTCAAAACAAAACGA
TGAGGCAGCGAAAAGAAATAGAGTTGTCAGTCCATTTGGATTGTCATTTCAATGGGGGATAT
TTAAACCCATCCAATATCGAGTAACAATTGGAGGACAAGTCTGGTGCCAGCACCCGCGGTAA
TTCCAGCTCCAAAAGCGTATATTAATGCTGTTGCTGTTAAAGGGTTCGTAGTTGAACTGTGG
GCTGTGCAGGTTTGTTCCTGGTCGTCCCGTCCATGTCGGATTTGGTGACCCAGGCCCTTGC
AGCCCGTGAACATTCAAAGAAACAAGAAACACGGGAGTGGTTCCTTTCCTGATTTACGCATG
TCATGCATGCGTCGAGGATCCAGTCGCAGCCTGACCGCATCACACATCAAGGCGTTACAGC
CTCTCCGTCTTTGCTGCACCCAAGTGTATTCGTGTGACCGCGTTGAGGGATCTAATGGATTC
GGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCCGTGCCTCTC
TGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGCGTGCGCCATCTGACAA
CGAATAGATCAGCAGCATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTA
TTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGC
TCTTCACGCTCCTGCTTTCCTTGCTGTGCCTTGCCACCAGATCTGCCATGGCCTCGAGGCTC
GTCCGTGTGCTGGCCGCCGCCATGCTGGTTGCAGCGGCCGTGTCGGTCGACGCTGGCGCC
TCTCTAGACACAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGT
GCTCGAGAAGAATGTGACAGTGACACACTCTGTTAACCTGCTCGAAGACAGCCACAACGGA
AAACTATGTAGATTAAAAGGAATAGCCCCACTACAATTGGGGAAATGTAACATCGCCGGATG
GCTCTTGGGAAACCCAGAATGCGACCCACTGCTTCCAGTGAGATCATGGTACTACATTGTAG
AAACACCAAACTCTGAGAATGGAATATGTTATCCAGGAGATTTCATCGACTATGAGGAGCTG
AGGGAGCAATTGAGCTCAGTGTCATCATTCGAAAGATTCGAAATATTTCCCAAAGAAAGCTC
ATGGCCCAACCACAACACAAACAAAGGAGTAACGGCAGCATGCTCCCATGCGGGGGAAAGC
AGTTTTTACAGAAATTTGCTATGGCTGACGGAGAAGGAGGGCTCATACCCAAAGCTGAAAAA
TTCTTATGTGAACAAGAGAGGGAAAGAAGTCCTTGTACTGTGGGGTATTCATCACCCGTCTA
ACAGTAAGGAGCAACAGAATATCTATCAGAATGAAAATGCTTATGTCTCTGTAGTGACTTCAA
ATTATAACAGGAGATTTACCCCGGAAATAGCAGAAAGACCCAAAGTAAGAGATCAAGCTGGG
AGGATGAACTATTACTGGACCTTGCTAAAACCCGGAGACACAATAATATTTGAGGCAAATGG
AAATCTAATAGCACCAAGGTATGCTTTCGCACTGAGTAGAGGCTTTGGGTCCGGCATCATCA
CCTCAAACGCATCAATGCATGAGTGTAACACGAAGTGTCAAACACCCCTGGGAGCTATAAAC
AGCAGTCTCCCTTTCCAGAATATACACCCAGTCACAATAGGAGAGTGCCCAAAATACGTCAG
GAGTGCCAAATTGAGGATGGTTACAGGACTAAGGAACATTCCGTCCATTCAATCCGCTAGCC
TTAAGGGTACCCACCACCATCACCACCACTAGGCGGCCGCCCTCCTCCTCCTTTCTTGTTCC
TTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGACGAGTCTTACTTTTACGTGTACTTCT
CTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATGTGTCCGTGTGTTGTGTACG
CGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCATGATGCGTGTGTATA
TCCACGCGCAGGCACGCACACACACACACACACACACACACACAGGCACACACAGGCACAC
AAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGATCATGTAG
TTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAAGCCCG
ATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGGA
GGGGGGAAGGGAGGCATAAACGGATCGATCCCCCATCGCACATGCGGGTACGAGCATTAT
CTGCTGTGTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCC
CCCCCCCGCGGCTCCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCA
TGTCACGAAGAGAAAAGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGC
ATGCAGACGTCGAGCCGTGGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAG
GAGATTTACTGCAGGACGTCTACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAG
GAAAATTGGGGGGGAGGGCGCACGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACT
CTCAACTTGTGTCCGTATTGCGAAGGAGGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAA
ACGTCGGAACCACCTATGAATCCCACTTCTGCGTCGCGGAGGCGTACTGCTTCCGACCACC
TCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTTCCCCTTCTTTCCGTTGTGTGTGTGTGTG
TGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCAACCCTCCACGCCTTCCTCTCCC
CCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTATCACATCCACCACCACTCT
TACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCCGCCTCTCTCGCTCG
CTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCCCACCCACCCACCCA
CCCACCCACCGCTCTACACACCTCCACAGCAGCCGGATCCAATATGGGATCGGCCATTGAA
CAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACT
GGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGC
GCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGC
AGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGT
CACTGAAGCGGGAAGGGACTGGTTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTC
ATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATA
CGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACG
TACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC
GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTC
GTGACGCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATT
CATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCG
TGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATC
GCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAACTAG
TTCTAGTTCTAGGGGCCGAATTCAGATCCTCGTGTGAGCGTTCGCGGAATCGGTCGCTCGT
GTTTATGCCCGTCTTGGTGTTGTGCTCGCAAGGCGGTGCAGCAGGATACCGTCGCCCTCCT
CTCTCCTTGCTTCTCTGTTCTTCAATTCGCGATCTCACAGAGGCCGGCTGTGCACGCCCTTC
CTCACCCTCCTTTTCCCACCTCTCGGCCACCGGTCGGCTCCGTTCCGTCTGCCGTCGAGAA
GGGACGGGCATGTGCAGCTCCTCCCTTTCTCTCGCGCGCGCATCTTCTCTTGCTTGTGGCA
CTCACGCTCATGCGTCAAGGCGGCCCCACGCCGAGCCCCTGCGCTCCCTTCCCTCTTGCG
CATCCGTAGCGCGGATGCCGTCGATGCGCAAGGCCGGCATGAAGGAGCGCGTGCCCTCAA
GAGGGCACACTATCATGCCCTACGTGGGCCACGCAGCGATGAGGCCGGCTTCGGCGGAGA
TGCGTCACGCACGTGCCAGATGATGCGCTACGCCTTCCTTGACTTGCGCCCCCCTCTCTTC
CTCCGTCTCTCACTCTCTCTCTCTCACACACACACACACACACACACACACACAAAGCTCCG
GTTCGTCCGGCCGTAACGCCTTTTCAACTCACGGCCTCTAGGAATGAAGGAGGGTAGTTCG
GGGGAGAACGTACTGGGGCGTCAGAGGTGAAATTCTTAGACCGCACCAAGACGAACTACAG
CGAAGGCATTCTTCAAGGATACCTTCCTCAATCAAGAACCAAAGTGTGGAGATCGAAGATGA
TTAGAGACCATTGTAGTCCACACTGCAAACGATGACACCCATGAATTGGGGATCTTATGGGC
CGGCCTGCGGCAGGGTTTACCCTGTGTCAGCACCGCGCCCGCTTTTACCAACTTACGTATC
TTTTCTATTCGGCCTTTACCGGCCACCCACGGGAATATCCTCAGCACGTTTTCTGTTTTTTCA
CGCGAAAGCTTTGAGGTTACAGTCTCAGGGGGGAGTACGTTCGCAAGAGTGAAACTTAAAG
AAATTGACGGAATGGCACCACAAGACGTGGAGCGTGCGGTTTAATTTGACTCAACACGGGG
AACTTTACCAGATCCGGACAGGATGAGGATTGACAGATTGAGTGTTCTTTCTCGATTCCCTG
AATGGTGGTGCATGGCCGCTTTTGGTCGGTGGAGTGATTTGTTTGGTTGATTCCGTCAACGG
ACGAGATCCAAGCTGCCCAGTAGAATTCAGAATTGCCCATAGAATAGCAAACTCATCGGCGG
GTTTTACCCAACGGTGGGCCGCATTCGGTCGAATTCTTCTCTGCGGGATTCCTTTGTAATTG
CACAAGGTGAAATTTTGGGCAACAGCAGGTCTGTGATGCTCCTCAATGTTCTGGGCGACAC
GCGCACTACAATGTCAGTGAGAACAAGAAAAACGACTTTTGTCGAACCTACTTGATCAAAAG
AGTGGGGAAACCCCGGAATCACATAGACCCACTTGGGACCGAGGATTGCAATTATTGGTCG
CGCAACGAGGAATGTCTCGTAGGCGCAGCTCATCAAACTGTGCCGATTACGTCCCTGCCAT
TTGTACACACCGCCCGTCGTTGTTTCCGATGATGGTGCAATACAGGTGATCGGACAGGCGG
TGTTTTATCCGCCCGAAAGTTCACCGATATTTAAATCCAGCTTTTGTTCCCTTTAGTGAGGGT
TAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCA
CAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT
GAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGT
GCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCT
CTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC
ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCG
AAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT
CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG
GGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT
TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGC
TACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAG
AGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGG
TCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG
GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAG
TAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTA
TTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTT
ACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTA
TCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCG
CCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTT
TGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCT
TCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAA
AGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCAC
TCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTG
TGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCT
TGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCAT
TGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA
TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGT
GAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG
AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGC
GGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGA
AAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACG
CGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTT
CCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCGCCCTTTAGG
GTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCAC
GTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTT
AATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGAT
TTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAAATGAGCTGATTTAACAAAAATTT
AACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACT
GTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT
GTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAAC
GACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGATTT