pLEXSY-egfp-sat2 Cat-No. EGE-235 Description: nt 0001-0686: 5īpart of 18S rRNA of Leishmania nt 0693-1079: 5īnon-translated region of aprt gene of Leishmania with splice acceptor site for target gene nt 0990-1009: annealing sequencing forward primer P1442 nt 1089-1808: egfp control gene nt 1818-3152: 1.4k intergenic region from cam operon of Leishmania with polyA site for target gene and splice acceptor site for marker gene nt 1885-1910: annealing sequencing reverse primer A264 (marked in yellow) nt 3163-3687: sat marker gene nt 3737-4310: 5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene nt 4311-5395: 3īpart of 18S rRNA of Leishmania nt 5436-8218: pBluescript II KS (-) with ori and bla marker gene @ 6613-7473 AAATTGGATAACTTGGCGAAACGCCAAGCTAATACATGAACCAACCGGGTGTTCTCCACTCCAGA CGGTGGGCAACCATCGTCGTGAGACGCCCAGCGAATGAATGACAGTAAAACCAATGCCTTCACT GGCAGTAACACCCAGCAGTGTTGACTCAATTCATTCCGTGCGAAAGCCGGCTTGTTCCGGCGTCT TTTGACGAACAACTGCCCTATCAGCTGGTGATGGCCGTGTAGTGGACTGCCATGCATGGCGTTGA CGGGAGCGGGGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACTTCTACG GAGGGCAGCAGGCGCGCAAATTGCCCAATGTCAAAACAAAACGATGAGGCAGCGAAAAGAAAT AGAGTTGTCAGTCCATTTGGATTGTCATTTCAATGGGGGATATTTAAACCCATCCAATATCGAGTAA CAATTGGAGGACAAGTCTGGTGCCAGCACCCGCGGTAATTCCAGCTCCAAAAGCGTATATTAATG CTGTTGCTGTTAAAGGGTTCGTAGTTGAACTGTGGGCTGTGCAGGTTTGTTCCTGGTCGTCCCGTC CATGTCGGATTTGGTGACCCAGGCCCTTGCAGCCCGTGAACATTCAAAGAAACAAGAAACACGG GAGTGGTTCCTTTCCTGATTTACGCATGTCATGCATGCGTCGACAGTCGCAGCCTGACCGCATCAC ACATCAAGGCGTTACAGCCTCTCCGTCTTTGCTGCACCCAAGTGTATTCGTGTGACCGCGTTGAGG GATCTAATGGATTCGGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCC GTGCCTCTCTGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGCGTGCGCCATCTG ACAACGAATAGATCAGCAGCATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTA TTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGCTCTT CACGCTCCTGCTTTCCTTGCTGTGCCTTTCCACCAGATCTGCCATGGTGAGCAAGGGCGAGGAGC TGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAG CGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCAC CACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGC TTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTA CGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAA GTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGG CAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGAC AAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTG CAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGAC AACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATG GTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAA GCGGCCGCCCTCCTCCTCCTTTCTTGTTCCTTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGAC GAGTCTTACTTTTACGTGTACTTCTCTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATG TGTCCGTGTGTTGTGTACGCGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCAT GATGCGTGTGTATATCCACGCGCAGGCACGCACACACACACACACACACACACACACAGGCACA CACAGGCACACAAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGA TCATGTAGTTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAA GCCCGATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGG AGGGGGGAAGGGAGGCATAAACGGATCCCCCATCGCACATGCGGGTACGAGCATTATCTGCTGT GTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCCCCCCCCCGCGG CTCCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCATGTCACGAAGAGAAA AGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGCATGCAGACGTCGAGCCGT GGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAGGAGATTTACTGCAGGACGTCT ACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAGGAAAATTGGGGGGGAGGGCGCA CGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACTCTCAACTTGTGTCCGTATTGCGAAGGA GGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAAACGTCGGAACCACCTATGAATCCCACTTCT GCGTCGCGGAGGCGTACTGCTTCCGACCACCTCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTT CCCCTTCTTTCCGTTGTGTGTGTGTGTGTGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCA ACCCTCCACGCCTTCCTCTCCCCCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTAT CACATCCACCACCACTCTTACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCC GCCTCTCTCGCTCGCTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCCCACC CACCCACCCACCCACCCACCGCTCTACACACCTCCACAGCAGCCAGATCCTAGTATGAAGATTTC GGTGATCCCTGAGCAGGTGGCGGAAACATTGGATGCTGAGAACCATTTCATTGTTCGTGAAGTGT TCGATGTGCACCTATCCGACCAAGGCTTTGAACTATCTACCAGAAGTGTGAGCCCCTACCGGAAG GATTACATCTCGGATGATGACTCTGATGAAGACTCTGCTTGCTATGGCGCATTCATCGACCAAGAG CTTGTCGGGAAGATTGAACTCAACTCAACATGGAACGATCTAGCCTCTATCGAACACATTGTTGTG TCGCACACGCACCGAGGCAAAGGAGTCGCGCACAGTCTCATCGAATTTGCGAAAAAGTGGGCAC TAAGCAGACAGCTCCTTGGCATACGATTAGAGACACAAACGAACAATGTACCTGCCTGCAATTTG TACGCAAAATGTGGCTTTACTCTCGGCGGCATTGACCTGTTCACGTATAAAACTAGACCTCAAGTC TCGAACGAAACAGCGATGTACTGGTACTGGTTCTCGGGAGCACAGGATGACGCCTAACTAGCCT CGGAGATCCACTAGTTCTAGTTCTAGGGGCCGAATTCAGATCCTCGTGTGAGCGTTCGCGGAATC GGTCGCTCGTGTTTATGCCCGTCTTGGTGTTGTGCTCGCAAGGCGGTGCAGCAGGATACCGTCGC CCTCCTCTCTCCTTGCTTCTCTGTTCTTCAATTCGCGATCTCACAGAGGCCGGCTGTGCACGCCCTT CCTCACCCTCCTTTTCCCACCTCTCGGCCACCGGTCGGCTCCGTTCCGTCTGCCGTCGAGAAGGG ACGGGCATGTGCAGCTCCTCCCTTTCTCTCGCGCGCGCATCTTCTCTTGCTTGTGGCACTCACGCT CATGCGTCAAGGCGGCCCCACGCCGAGCCCCTGCGCTCCCTTCCCTCTTGCGCATCCGTAGCGC GGATGCCGTCGATGCGCAAGGCCGGCATGAAGGAGCGCGTGCCCTCAAGAGGGCACACTATCA TGCCCTACGTGGGCCACGCAGCGATGAGGCCGGCTTCGGCGGAGATGCGTCACGCACGTGCCA GATGATGCGCTACGCCTTCCTTGACTTGCGCCCCCCTCTCTTCCTCCGTCTCTCACTCTCTCTCTCTC ACACACACACACACACACACACACACACAAAGCTCCGGTTCGTCCGGCCGTAACGCCTTTTCAAC TCACGGCCTCTAGGAATGAAGGAGGGTAGTTCGGGGGAGAACGTACTGGGGCGTCAGAGGTGA AATTCTTAGACCGCACCAAGACGAACTACAGCGAAGGCATTCTTCAAGGATACCTTCCTCAATCAA GAACCAAAGTGTGGAGATCGAAGATGATTAGAGACCATTGTAGTCCACACTGCAAACGATGACA CCCATGAATTGGGGATCTTATGGGCCGGCCTGCGGCAGGGTTTACCCTGTGTCAGCACCGCGCC CGCTTTTACCAACTTACGTATCTTTTCTATTCGGCCTTTACCGGCCACCCACGGGAATATCCTCAGC ACGTTTTCTGTTTTTTCACGCGAAAGCTTTGAGGTTACAGTCTCAGGGGGGAGTACGTTCGCAAGA GTGAAACTTAAAGAAATTGACGGAATGGCACCACAAGACGTGGAGCGTGCGGTTTAATTTGACTC AACACGGGGAACTTTACCAGATCCGGACAGGATGAGGATTGACAGATTGAGTGTTCTTTCTCGATT CCCTGAATGGTGGTGCATGGCCGCTTTTGGTCGGTGGAGTGATTTGTTTGGTTGATTCCGTCAACG GACGAGATCCAAGCTGCCCAGTAGAATTCAGAATTGCCCATAGAATAGCAAACTCATCGGCGGG TTTTACCCAACGGTGGGCCGCATTCGGTCGAATTCTTCTCTGCGGGATTCCTTTGTAATTGCACAAG GTGAAATTTTGGGCAACAGCAGGTCTGTGATGCTCCTCAATGTTCTGGGCGACACGCGCACTACA ATGTCAGTGAGAACAAGAAAAACGACTTTTGTCGAACCTACTTGATCAAAAGAGTGGGGAAACCC CGGAATCACATAGACCCACTTGGGACCGAGGATTGCAATTATTGGTCGCGCAACGAGGAATGTCT CGTAGGCGCAGCTCATCAAACTGTGCCGATTACGTCCCTGCCATTTGTACACACCGCCCGTCGTT GTTTCCGATGATGGTGCAATACAGGTGATCGGACAGGCGGTGTTTTATCCGCCCGAAAGTTCACC GATATTTAAATCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCAT AGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAA AGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAG GCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGC TGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT GCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCG CTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC GCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAA AAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAAT GAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAG TGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAG ATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCAC GCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC GCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTT TGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTG CAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATC ACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTG ACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCC GGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAAC GTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTC GTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAA GGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTT TTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAG AAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTA GCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCG CCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCA AGCTCTAAATCGGGGGCGCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAA AACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGA CGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTC GGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAAATGAGCTGATT TAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCT GCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAA CGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGATTT