pLEXSY-ble2 Cat-No. EGE-231

Description:

nt 1-686:		5«part of 18S rRNA of Leishmania 
nt 698-1085:		5«non-translated region of aprt gene of Leishmania 
			with splice acceptor site for target gene
nt 1085-1104: 		MCS
nt 1094-1162 :		signal peptide LMSAP
nt 1154-1176 :		MCS  
nt 1177-2152:		stuffer
nt 2153-2170:		MCS
nt 2200-3538:		1.4k intergenic region from cam operon of Leishmania 
with polyA site for target gene and splice acceptor site for marker gene
nt 3550-3924:		ble marker gene
nt 3961-4534:	 5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene
nt 4535-5619:		3«part of 18S rRNA of Leishmania
nt 5624-8480:		pBluescript II KS (-) with ori and bla marker gene @ 6837-7697


> pLEXSY-ble2 Cat-No. EGE-231
AAATTGGATAACTTGGCGAAACGCCAAGCTAATACATGAACCAACCGGGTGTTCTCCACTCC
AGACGGTGGGCAACCATCGTCGTGAGACGCCCAGCGAATGAATGACAGTAAAACCAATGCC
TTCACTGGCAGTAACACCCAGCAGTGTTGACTCAATTCATTCCGTGCGAAAGCCGGCTTGTT
CCGGCGTCTTTTGACGAACAACTGCCCTATCAGCTGGTGATGGCCGTGTAGTGGACTGCCA
TGCATGGCGTTGACGGGAGCGGGGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA
TAGCTACCACTTCTACGGAGGGCAGCAGGCGCGCAAATTGCCCAATGTCAAAACAAAACGA
TGAGGCAGCGAAAAGAAATAGAGTTGTCAGTCCATTTGGATTGTCATTTCAATGGGGGATAT
TTAAACCCATCCAATATCGAGTAACAATTGGAGGACAAGTCTGGTGCCAGCACCCGCGGTAA
TTCCAGCTCCAAAAGCGTATATTAATGCTGTTGCTGTTAAAGGGTTCGTAGTTGAACTGTGG
GCTGTGCAGGTTTGTTCCTGGTCGTCCCGTCCATGTCGGATTTGGTGACCCAGGCCCTTGC
AGCCCGTGAACATTCAAAGAAACAAGAAACACGGGAGTGGTTCCTTTCCTGATTTACGCATG
TCATGCATGCGTCGAGGATCCAGTCGCAGCCTGACCGCATCACACATCAAGGCGTTACAGC
CTCTCCGTCTTTGCTGCACCCAAGTGTATTCGTGTGACCGCGTTGAGGGATCTAATGGATTC
GGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCCGTGCCTCTC
TGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGCGTGCGCCATCTGACAA
CGAATAGATCAGCAGCATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTA
TTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGC
TCTTCACGCTCCTGCTTTCCTTGCTGTGCCTTGCCACCAGATCTGCCATGGCCTCGAGGCTC
GTCCGTGTGCTGGCCGCCGCCATGCTGGTTGCAGCGGCCGTGTCGGTCGACGCTGGCGCC
TCTCTAGACACAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGT
GCTCGAGAAGAATGTGACAGTGACACACTCTGTTAACCTGCTCGAAGACAGCCACAACGGA
AAACTATGTAGATTAAAAGGAATAGCCCCACTACAATTGGGGAAATGTAACATCGCCGGATG
GCTCTTGGGAAACCCAGAATGCGACCCACTGCTTCCAGTGAGATCATGGTACTACATTGTAG
AAACACCAAACTCTGAGAATGGAATATGTTATCCAGGAGATTTCATCGACTATGAGGAGCTG
AGGGAGCAATTGAGCTCAGTGTCATCATTCGAAAGATTCGAAATATTTCCCAAAGAAAGCTC
ATGGCCCAACCACAACACAAACAAAGGAGTAACGGCAGCATGCTCCCATGCGGGGGAAAGC
AGTTTTTACAGAAATTTGCTATGGCTGACGGAGAAGGAGGGCTCATACCCAAAGCTGAAAAA
TTCTTATGTGAACAAGAGAGGGAAAGAAGTCCTTGTACTGTGGGGTATTCATCACCCGTCTA
ACAGTAAGGAGCAACAGAATATCTATCAGAATGAAAATGCTTATGTCTCTGTAGTGACTTCAA
ATTATAACAGGAGATTTACCCCGGAAATAGCAGAAAGACCCAAAGTAAGAGATCAAGCTGGG
AGGATGAACTATTACTGGACCTTGCTAAAACCCGGAGACACAATAATATTTGAGGCAAATGG
AAATCTAATAGCACCAAGGTATGCTTTCGCACTGAGTAGAGGCTTTGGGTCCGGCATCATCA
CCTCAAACGCATCAATGCATGAGTGTAACACGAAGTGTCAAACACCCCTGGGAGCTATAAAC
AGCAGTCTCCCTTTCCAGAATATACACCCAGTCACAATAGGAGAGTGCCCAAAATACGTCAG
GAGTGCCAAATTGAGGATGGTTACAGGACTAAGGAACATTCCGTCCATTCAATCCGCTAGCC
TTAAGGGTACCCACCACCATCACCACCACTAGGCGGCCGCCCTCCTCCTCCTTTCTTGTTCC
TTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGACGAGTCTTACTTTTACGTGTACTTCT
CTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATGTGTCCGTGTGTTGTGTACG
CGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCATGATGCGTGTGTATA
TCCACGCGCAGGCACGCACACACACACACACACACACACACACAGGCACACACAGGCACAC
AAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGATCATGTAG
TTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAAGCCCG
ATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGGA
GGGGGGAAGGGAGGCATAAACGGATCGATCCCCCATCGCACATGCGGGTACGAGCATTAT
CTGCTGTGTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCC
CCCCCCCGCGGCTCCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCA
TGTCACGAAGAGAAAAGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGC
ATGCAGACGTCGAGCCGTGGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAG
GAGATTTACTGCAGGACGTCTACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAG
GAAAATTGGGGGGGAGGGCGCACGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACT
CTCAACTTGTGTCCGTATTGCGAAGGAGGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAA
ACGTCGGAACCACCTATGAATCCCACTTCTGCGTCGCGGAGGCGTACTGCTTCCGACCACC
TCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTTCCCCTTCTTTCCGTTGTGTGTGTGTGTG
TGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCAACCCTCCACGCCTTCCTCTCCC
CCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTATCACATCCACCACCACTCT
TACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCCGCCTCTCTCGCTCG
CTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCCCACCCACCCACCCA
CCCACCCACCGCTCTACACACCTCCACAGCAGCCGGATCCACCGCATGGCCAAGTTGACCA
GTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACC
GGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACG
TGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGG
TGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACT
TCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAG
TTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA
CCGACTAGTTCTAGTTCTAGGGGCCGAATTCAGATCCTCGTGTGAGCGTTCGCGGAATCGG
TCGCTCGTGTTTATGCCCGTCTTGGTGTTGTGCTCGCAAGGCGGTGCAGCAGGATACCGTC
GCCCTCCTCTCTCCTTGCTTCTCTGTTCTTCAATTCGCGATCTCACAGAGGCCGGCTGTGCA
CGCCCTTCCTCACCCTCCTTTTCCCACCTCTCGGCCACCGGTCGGCTCCGTTCCGTCTGCC
GTCGAGAAGGGACGGGCATGTGCAGCTCCTCCCTTTCTCTCGCGCGCGCATCTTCTCTTGC
TTGTGGCACTCACGCTCATGCGTCAAGGCGGCCCCACGCCGAGCCCCTGCGCTCCCTTCC
CTCTTGCGCATCCGTAGCGCGGATGCCGTCGATGCGCAAGGCCGGCATGAAGGAGCGCGT
GCCCTCAAGAGGGCACACTATCATGCCCTACGTGGGCCACGCAGCGATGAGGCCGGCTTC
GGCGGAGATGCGTCACGCACGTGCCAGATGATGCGCTACGCCTTCCTTGACTTGCGCCCC
CCTCTCTTCCTCCGTCTCTCACTCTCTCTCTCTCACACACACACACACACACACACACACACA
AAGCTCCGGTTCGTCCGGCCGTAACGCCTTTTCAACTCACGGCCTCTAGGAATGAAGGAGG
GTAGTTCGGGGGAGAACGTACTGGGGCGTCAGAGGTGAAATTCTTAGACCGCACCAAGACG
AACTACAGCGAAGGCATTCTTCAAGGATACCTTCCTCAATCAAGAACCAAAGTGTGGAGATC
GAAGATGATTAGAGACCATTGTAGTCCACACTGCAAACGATGACACCCATGAATTGGGGATC
TTATGGGCCGGCCTGCGGCAGGGTTTACCCTGTGTCAGCACCGCGCCCGCTTTTACCAACT
TACGTATCTTTTCTATTCGGCCTTTACCGGCCACCCACGGGAATATCCTCAGCACGTTTTCTG
TTTTTTCACGCGAAAGCTTTGAGGTTACAGTCTCAGGGGGGAGTACGTTCGCAAGAGTGAAA
CTTAAAGAAATTGACGGAATGGCACCACAAGACGTGGAGCGTGCGGTTTAATTTGACTCAAC
ACGGGGAACTTTACCAGATCCGGACAGGATGAGGATTGACAGATTGAGTGTTCTTTCTCGAT
TCCCTGAATGGTGGTGCATGGCCGCTTTTGGTCGGTGGAGTGATTTGTTTGGTTGATTCCGT
CAACGGACGAGATCCAAGCTGCCCAGTAGAATTCAGAATTGCCCATAGAATAGCAAACTCAT
CGGCGGGTTTTACCCAACGGTGGGCCGCATTCGGTCGAATTCTTCTCTGCGGGATTCCTTT
GTAATTGCACAAGGTGAAATTTTGGGCAACAGCAGGTCTGTGATGCTCCTCAATGTTCTGGG
CGACACGCGCACTACAATGTCAGTGAGAACAAGAAAAACGACTTTTGTCGAACCTACTTGAT
CAAAAGAGTGGGGAAACCCCGGAATCACATAGACCCACTTGGGACCGAGGATTGCAATTAT
TGGTCGCGCAACGAGGAATGTCTCGTAGGCGCAGCTCATCAAACTGTGCCGATTACGTCCC
TGCCATTTGTACACACCGCCCGTCGTTGTTTCCGATGATGGTGCAATACAGGTGATCGGACA
GGCGGTGTTTTATCCGCCCGAAAGTTCACCGATATTTAAATCCAGCTTTTGTTCCCTTTAGTG
AGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATC
CGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTA
ATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACC
TGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGG
GCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGC
GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGA
AAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAG
GTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG
CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAA
CAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC
TACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGG
AAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCA
AAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATAT
ATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATC
TGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA
GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCA
GATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTT
TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTT
AATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGG
TATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGT
GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGT
GTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATG
CTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGA
GTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTG
CTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATC
CAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGT
TTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGG
AAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCT
CATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT
TCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGT
GGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTC
TTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCGCC
CTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGAT
GGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCA
CGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATT
CTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAAATGAGCTGATTTAAC
AAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTG
CGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAA
GGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTT
GTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGATTT