Catalog Standard PCR - Core KitsStandard PCR - Core Kits
 
 
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Direct PCR and Multiplex Kits   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   dNTP Mixes   |   dNTP Bundles and Single Solutions   |   Supplements   |   School and Demo Kits
 
Standard PCR Core Kits
Complete sets of reagents required for PCR

Core Kits provide you premium quality enzymes, ultrapure dNTPs and optimized reaction buffers. Each kit contains complete reaction buffer with MgCl2 ensuring superior results in a broad range of reaction conditions or template-primer conditions. The additional reaction buffer without MgCl2 in combination with the MgCl2 stock solution allows the optimization of magnesium-sensitive PCR reactions. The kit combines simple handling with high flexibility.

Standard PCR - Product Selection Guide
 

Taq Core Kit
Kit of thermostable DNA polymerase, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-232S 200 units 48,00    Add this product to your notepad  
L pack PCR-232L 1000 units 192,00    Add this product to your notepad
red - S pack PCR-232S-red 200 units 48,00    Add this product to your notepad
red - L pack PCR-232L-red 1000 units 192,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Taq Pol (5 units/l)
dNTP Mix
Taq Reaction Buffer complete - 10 x conc.
MgCl2 Stock Solution
Taq Core Kit is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The buffer system is recommended for plate based PCR and automated pipetting.
Taq Core Kit - red contains Taq Pol - red for easier handling and to facilitate the preparation of the master mix.
 
Taq Core Kit / high yield
Kit of thermostable DNA polymerase, dNTPs and high yield buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-231S 200 units 48,00    Add this product to your notepad  
L pack PCR-231L 1000 units 192,00    Add this product to your notepad
red - S pack PCR-231S-red 200 units 48,00    Add this product to your notepad
red - L pack PCR-231L-red 1000 units 192,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Taq Pol (5 units/l)
dNTP Mix
High Yield Buffer complete - 10 x conc.
MgCl2 Stock Solution
Taq Core Kit / high yield is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives best results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
Taq Core Kit - red / high yield contains Taq Pol - red for easier handling and to facilitate the preparation of the master mix.
 
Hot Start Core Kit
Kit of heat-activatable DNA polymerase for high specificity, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-233S 200 units 83,00    Add this product to your notepad  
L pack PCR-233L 1000 units 332,00    Add this product to your notepad
red - S pack PCR-233S-red 200 units 83,00    Add this product to your notepad
red - L pack PCR-233L-red 1000 units 332,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Hot Start Pol (5 units/l)
dNTP Mix
Hot Start Buffer complete - 10 x conc.
MgCl2 Stock Solution
Hot Start Core Kit provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
Hot Start Pol requires no prolonged activation step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Hot Start Core Kit - red contains Hot Start Pol - red for easier handling and to facilitate the preparation of the master mix.
 
Rapid PCR Core Kit
Kit of rapid DNA polymerase, dNTPs and Rapid Buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-238S 100 units 82,00    Add this product to your notepad  
L pack PCR-238L 500 units 328,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Rapid Pol
dNTP Mix
Rapid Buffer - 5 x conc.
MgCl2 Stock Solution
Rapid PCR Core Kit contains a specifically processed enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase.
The rapid polymerase is designed for Fast PCR, in which total reaction times are 20-70% shorter compared with conventional PCR assays. This can be achieved without sacrificing reaction performance or the requirement for specialized PCR consumables or thermocyclers.
 
High Fidelity Core Kit
Kit of thermostable DNA polymerase for high accuracy, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-234S 100 units 56,00    Add this product to your notepad  
L pack PCR-234L 500 units 224,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Pol (2.5 units/l)
dNTP Mix
High Fidelity Buffer - 10 x conc.
High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.
 
High Fidelity Hot Start Core Kit
Kit of heat-activatable DNA polymerase for high accuracy and specificity, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-235S 100 units 76,00    Add this product to your notepad  
L pack PCR-235L 500 units 304,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Hot Start Pol (2.5 units/l)
dNTP Mix
High Fidelity Buffer - 10 x conc.
High Fidelity Hot Start Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.
 
Pfu-X Core Kit
Kit of proofreading DNA polymerase for highest accuracy, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-237S 100 units 68,00    Add this product to your notepad  
L pack PCR-237L 500 units 272,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Pfu-X Polymerase (2.5 units/l)
dNTP Mix
Pfu-X Buffer - 10 x conc.
Pfu-X Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.