Catalog evoglow® - Anaerobic Fluorescent Proteins - evoglow® Plasmid Kits
 
 
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evoglow® Plasmid Kits   |   evoglow® Antibodies
 

evoglow® Basic Kit

The basic kit was composed for easy cloning of the evoglow®-genes into the customer-specific host- and vector systems. It comprises three different variants of evoglow®-proteins, either with and without a STOP-codon (6 plasmids) on high-copy plasmids. The plasmids replicate well in E. coli and are ideal templates for PCR. They furthermore bear a variety of restriction sites for direct cloning in specific vector-systems.
 

evoglow® Express Kit

The evoglow® express kit comprises a variety of plasmids designed for efficient expression in a wide host range of gram-negative host organisms. The proteins can be expressed either under control of the inducible T7-promotor as well as in a constiutive manner (kan-promotor).
 

evoglow® Fusion Kit

The evoglow® fusion kit was designed to create transcriptional protein fusions. It contains plasmids on which the evoglow®-gene are flanked by symmetrical multiple cloning sites (MCS) which enables cloning via a singular restriction site i.e. for conducting promotor activity studies or further physiological investigations.
 

evoglow® Yeast Kit

The evoglow® yeast kit was created to enable expression of the fluorescent proteins in yeasts and related organisms. It comprises plasmids on which the evoglow® genes are controlled by different yeast promotors (Gal and Act) and you can choose between plasmids that contain a single evoglow® gene or a fusion of two genes per plasmid.
 

evoglow® Clostridia Kit

The evoglow clostridia kit was designed to express evoglow in Clostridia. It comprises the plasmids pGlow-C-Bs2 (Bacillus subtilis) and pGlow-C-Pp1 (Pseudomonas putida) for Gram-positive and Gram-negative bacteria respectively. The genes are cloned SacI / BamH1 into multiple cloning site of pUCC18 allowing individual subcloning under the control of the promotor of your choice.
 

Please contact us at info@jenabioscience.com for codon usage optimized variants of evoglow fluorescent proteins.
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
evoglow® Basic Kit FP-21010 1 Kit 1.250,00    Add this product to your notepad  
pGLOW-Bs1 FP-21011 2 Plasmids 450,00    Add this product to your notepad  
pGLOW-Bs2 FP-21012 2 Plasmids 450,00    Add this product to your notepad  
pGLOW-Pp1 FP-21013 2 Plasmids 450,00    Add this product to your notepad  
evoglow® Express Kit FP-21020 1 Kit 2.500,00    Add this product to your notepad  
evoglow® ExpressTN Kit FP-21021 1 Kit 1.250,00    Add this product to your notepad  
evoglow® ExpressKN Kit FP-21022 1 Kit 1.250,00    Add this product to your notepad  
pGLOW-TXN-Bs1 FP-21023 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-TXN-Bs2 FP-21024 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-TXN-Pp1 FP-21025 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-KXN-Bs1 FP-21026 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-KXN-Bs2 FP-21027 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-KXN-Pp1 FP-21028 1 Plasmid 450,00    Add this product to your notepad  
evoglow® Fusion Kit FP-21030 1 Kit 1.250,00    Add this product to your notepad  
pGLOW-FBs1 FP-21031 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-FBs2 FP-21032 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-FPp1 FP-21033 1 Plasmid 450,00    Add this product to your notepad  
evoglow® Yeast Kit 1+2 FP-21040 2 Kits 1.499,00    Add this product to your notepad  
evoglow® Yeast Kit 1 FP-21041 1 Kit 800,00    Add this product to your notepad  
evoglow® Yeast Kit 2 FP-21042 1 Kit 800,00    Add this product to your notepad  
pGLOW-ActXN-YT FP-21043 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-ActXN-YT-double FP-21044 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-GalXN-YT FP-21045 1 Plasmid 450,00    Add this product to your notepad  
pGLOW-GalXN-YT-double FP-21046 1 Plasmid 450,00    Add this product to your notepad  
evoglow® Clostridia Basic Kit FP-21060 1 Kit 880,00    Add this product to your notepad  
evoglow® Clostridia Expression Kit FP-21061 1 Kit 880,00    Add this product to your notepad  
evoglow® Whole Clostridia Kit FP-21062 1 Kit 1.499,00    Add this product to your notepad  
pGlow-C-Bs2 FP-21063 1 Plasmid 450,00    Add this product to your notepad  
pGlow-C-Pp1 FP-21064 1 Plasmid 450,00    Add this product to your notepad  
pGlow-CKXN-Bs2 FP-21065 1 Plasmid 450,00    Add this product to your notepad  
pGlow-CKXN-Pp1 FP-21066 1 Plasmid 450,00    Add this product to your notepad  

Selected References

  • Drepper et al. (2007) Reporter proteins for in vivo fluorescence without oxygen. Nature Biotech. 25 (4):443
  • Tielker et al. (2009) Flavin Mononucleotide-Based Fluorescent Protein as an Oxygen-Independent Reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryotic Cell 8 (6):913
  • Ernst et al. (2009) Responses to hypoxia in fungal pathogens. Cellular Microbiology 11 (2):183
  • Kensy et al. (2009) Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates. Microbial Cell Factories 8:31
  • Huber et al. (2009) Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content. Microbial Cell Factories 8:42

 
Please contact info@jenabioscience.com with questions or inquiries.