Glutathione S-transferase (GST) is a 26 kDa protein derived from Schistosoma japonicum. GST enzymes from various sources, both native and recombinantly expressed as fusion to the N-terminus of target proteins, are easily purified in a one-step procedure by affinity chromatography on immobilized glutathione (Glutathione Agarose, Fast Flow).

Due to the positive influence of the GST-tag on protein solubility and expression efficiency especially of small proteins, this technique has been widely used to generate proteins for crystallization, molecular immunology studies and studies involving protein-protein and protein-DNA interactions.

However, the GST-tag might sometimes interfere with these downstream applications. In these cases it is easily removed by protease cleavage e.g. by Factor Xa (GST Cleavage Capture Kit) provided that a specific protease sequence is located between the protein domain and GST.

Furthermore, free GST or GST fusion proteins are conveniently detected by Western blotting using GST antibody.

Glutathione Agarose, Fast Flow

High-performance 4 % agarose suitable for FPLC, gravity flow and batch purification of GST and GST tagged proteins.

GST Cleavage Capture Kit

The GST Cleavage Capture Kit allows the release and subsequent purification of free recombinant protein from their GST-tag provided that a Factor Xa recognition sequence is located between the protein domain and GST.

GST Antibody (ABD-035)

Affinity-purified rabbit polyclonal antibody raised against a recombinant full-length GST protein. It is suitable for the detection of GST and GST fusion proteins by Western Blot applications.