Catalog Standard PCR - Ready-to-Use MixesStandard PCR - Ready-to-Use Mixes
 
 
Direct PCR and Multiplex Kits   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   dNTP Mixes   |   dNTP Bundles and Single Solutions   |   Supplements   |   School and Demo Kits
 
Ready-to-Load Mixes
Ready-to-Use Mixes for Maximum Convenience

Ready-to-Use Mixes / direct gel loading contain an inherent dye and allow the direct loading of the PCR reaction product onto an agarose or acrylamide gel. The Taq master mixes are recommended for use in routine PCR reactions and ensure fast and easy preparation with a minimum of pipetting steps. The Hot start master mix is based on a heat-activatable Taq polymerase providing improved specifity and sensitivity. The mixes contain all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.

Standard PCR - Product Selection Guide
 

Red Load Taq Master
Master mix for direct gel loading
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-108S 100 reactions 45,00    Add this product to your notepad  
L pack PCR-108L 500 reactions 180,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Red Load Taq Master
PCR grade water
Red Load Taq Master is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The mix is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required.
 
Red Load Taq Master / high yield
Master mix for direct gel loading
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-106S 100 reactions 45,00    Add this product to your notepad  
L pack PCR-106L 500 reactions 180,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Red Load Taq Master / high yield
PCR grade water
Red Load Taq Master / high yield is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. Note that the mix is based on a detergent containing buffer system not recommended for plate based PCR and automated pipetting.
 
Red Load Hot Start Master
Heat-activatable master mix for direct gel loading
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-109S 100 reactions 90,00    Add this product to your notepad  
L pack PCR-109L 500 reactions 360,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Red Load Hot Start Master
PCR grade water
Red Load Hot Start Master contains an inherent red dye and allows the direct loading of the PCR reaction product onto the gel. It contains all reagents required for PCR (except template and primer) in a premixed 5x concentrated ready-to-use solution.
The master mix provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during PCR setup.
Activation step
Red Load Hot Start Master requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
 
Taq Master
Master mix of thermostable DNA polymerase
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-102S 100 reactions 40,00    Add this product to your notepad  
L pack PCR-102L 500 reactions 160,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Taq Master
PCR grade water
Taq Master is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation. The mix is particularly suitable for plate based PCR and automated pipetting where a detergent free buffer system is required.
 
Taq Master / high yield
Master mix of thermostable DNA polymerase
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-101S 100 reactions 40,00    Add this product to your notepad  
L pack PCR-101L 500 reactions 160,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Taq Master / high yield
PCR grade water
Taq Master / high yield is recommended for use in routine PCR reactions. It is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. Note that the mix is based on a detergent containing buffer system not recommended for plate based PCR and automated pipetting.
 
Hot Start Master
Master mix of heat-activatable DNA polymerase for high specificity
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-103S 100 reactions 80,00    Add this product to your notepad  
L pack PCR-103L 500 reactions 320,00    Add this product to your notepad

Store at 2 to 8C, if stored at -20C avoid frequent thawing and freezing

5x Hot Start Master
PCR grade water
Hot Start Master provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during PCR setup.
Activation step
Hot Start Master requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com