Catalog AntioxidantsAntioxidants
 
 

Oxidative stress

Free radicals emerge not only during normal oxygen metabolism and inflammatory processes, but also as a consequence of exposure to ionizing radiation, cigarette smoke or excessive alcohol consumption. Cellular free radicals target key organic substrates such as lipids, DNA and proteins. Protective agents and defense mechanisms act to minimize oxidative damage. This antioxidant system consists of (1) small molecules such as vitamin C and E, glutathione, carotenes and coenzyme Q, as well as (2) enzyme antioxidants which detoxify radicals or repair oxidized molecules. In aging cells and organisms, and under certain pathophysiological conditions - such as smokers’ emphysema, rheumatoid arthritis, Alzheimer’s and Parkinson’s disease - accumulation of biomolecule oxidation products indicate a disturbed balance in the oxidative stress response.

 

Product Accession No. Cat. No. Amount Price (EUR) Buy / Note Downloads
Cu/Zn SODHis
Cu/Zn Superoxide Dismutase
human, recombinant, E. coli
X02317 PR-150 500 µg 90,00    Add this product to your notepad  
Mn SODHis
Manganese Superoxide Dismutase
human, recombinant, E. coli
NP_000627 PR-149 500 µg 180,00    Add this product to your notepad  
MSRAHis
Methionine Sulfoxide Reductase A, EC1.8.4.6
human, recombinant, E. coli
NP_036463 PR-130 100 µg 180,00    Add this product to your notepad  
MSRBHis
Methionine-R-Sulfoxide Reductase B, EC1.8.4.6
E. coli, recombinant, E. coli
NP_416292 PR-132 100 µg 180,00    Add this product to your notepad  
MSRB2His
Methionine Sulfoxide Reductase B2, EC1.8.4.6
human, recombinant, E. coli
NP_036360 PR-131 100 µg 180,00    Add this product to your notepad  
MSRB3His
Methionine-R-Sulfoxide Reductase B3, EC1.8.4.6
human, recombinant, E. coli
AAH40053 PR-133 100 µg 180,00    Add this product to your notepad  
GST
Glutathione S-Transferase
Schistosoma japonicum, recombinant, E. coli
U58012 PR-811 10 µg 350,00    Add this product to your notepad  

Antioxidants
Superoxide Dismutases fight – together with Catalases - at the antioxidant forefront by detoxifying O2• radicals. In this way, these enzymes significantly decrease the free radical concentration in the cell and minimize oxidative damage. The metal-containing superoxide dismutases are located in mitochondria, the main cellular O2• production site. Decreased SOD activity may contribute to aging in cells, and mutations in the Cu/Zn-SOD are associated with neurodegenerative disease.


The side-chain sulfur of methionine is exceptionally susceptible towards oxidation, which may lead to inactivation and degradation of proteins affected. Methionine sulfoxide reductases A and B reduce the two stereoisomers of methionine sulfoxide (MetO), MetSO and MetRO, respectively, in a thioredoxin-dependent manner. Thus, they repair oxidized proteins. Since the oxidation of surface-exposed Met does not necessarily lead to inactivation, as shown for E. coli glutamine synthetase, cyclic oxidation by cellular oxidants followed by reduction by MSRs may also contribute to inactivation of free radicals.


MSRA and MSRB enzymes from Jena Bioscience constitute useful tools for oxidative stress research. Furthermore, MSRs can be utilized for the reduction of Met-containing peptides and proteins oxidized during synthesis or purification, with DTT efficiently substituting the thioredoxin system.

 
Also available: Antioxidant Antibodies
 
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