. qPCR Mixes for Dual Labeled Probes, Real-Time PCR, Molecular Biology, Products & Ordering, Jena Bioscience
 
 
Catalog Real-Time PCR - qPCR Mixes for Dual Labeled ProbesReal-Time PCR - qPCR Mixes for Dual Labeled Probes
 
 
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qPCR Multiplex Mixes   |   qPCR Mixes for Dual Labeled Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 
qPCR Master with ROX
qPCR Ready-to-Use Mixes for Dual Labeled Fluorescent Probes

JBS qPCR Master Series allows quantitative real-time analysis of DNA samples based on detection of labeled DNA probes. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.

Both qPCR ProbesMaster mixes with and without UNG (Uracil-N-Glycosylase) are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available. Mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan, Molecular Beacons or FRET probes.
 

qPCR ProbesMaster with UNG - clear
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-301S 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad  
L pack PCR-301L 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad

 
qPCR ProbesMaster with UNG - blue dyed
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-301S-bl 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad  
L pack PCR-301L-bl 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad

 
qPCR ProbesMaster
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-311S 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad  
clear - L pack PCR-311L 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad
blue dyed - S pack PCR-311S-bl 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad
blue dyed - L pack PCR-311L-bl 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible

qPCR ProbesMaster, 2x conc.
PCR-grade water
qPCR ProbesMaster is optimized for block-based PCR instruments.
 
qPCR ProbesMaster with UNG/lowROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-305S 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad  
clear - L pack PCR-305L 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad
blue dyed - S pack PCR-305S-bl 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad
blue dyed - L pack PCR-305L-bl 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with UNG/lowROX must be stored in the dark

qPCR ProbesMaster with UNG/lowROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with UNG/lowROX contains UNG (Uracil-N-Glycosylase) and 50 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with lowROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-315S 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad  
clear - L pack PCR-315L 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad
blue dyed - S pack PCR-315S-bl 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad
blue dyed - L pack PCR-315L-bl 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with lowROX must be stored in the dark

qPCR ProbesMaster with lowROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with lowROX contains 50 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with UNG/highROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-302S 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad  
clear - L pack PCR-302L 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad
blue dyed - S pack PCR-302S-bl 2x 1,25 ml
2x conc.
130,00    Add this product to your notepad
blue dyed - L pack PCR-302L-bl 10x 1,25 ml
2x conc.
520,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with UNG/highROX must be stored in the dark

qPCR ProbesMaster with UNG/highROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with UNG/highROX contains UNG (Uracil-N-Glycosylase) and 500 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with highROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-312S 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad  
clear - L pack PCR-312L 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad
blue dyed - S pack PCR-312S-bl 2x 1,25 ml
2x conc.
110,00    Add this product to your notepad
blue dyed - L pack PCR-312L-bl 10x 1,25 ml
2x conc.
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with highROX must be stored in the dark

qPCR ProbesMaster with highROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with highROX contains 500 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.