. . Inducible (Episomal), LEXSY Configurations, Eukaryotic Expression System LEXSY, Products & Ordering, Jena Bioscience
Catalog LEXSY Configurations - Inducible (Episomal)LEXSY Configurations - Inducible (Episomal)
Inducible (Genome integrated)   |   Inducible (Episomal)   |   Constitutive (Genome integrated)   |   Vectors   |   In Vitro (cell-free)   |   Custom Expression

The episomal inducible 4th generation of LEXSY makes use of amplification and oligomerisation of expression plasmids maintained extrachromosomally as self-replicating episomes in the LEXSY host cells, providing enhanced number of target gene copies for transcription by T7 RNA polymerase.

It was shown for a number of proteins, that the episomal inducible expression achieved 5 - 10-fold higher yields than integrative inducible expression. High expression strains are obtained by clonal selection and screening which is based on fluorescence intensity resulting from the co-expressed BleCherry, egfp or Ds-red genes. Primary screening on solid media can be performed in daylight since the colours are visible by eye.


Product Cat. No. Amount Price (EUR) Buy / Note Downloads
LEXSinduce4 Expression Kit
contains the episomal vector pLEXSY_IE-blecherry4
for inducible cytosolic or secretory protein expression
EGE-1420blecherry 1 Kit 2.250,00    Add this product to your notepad  
LEXSinduce4 Expression Kit
contains the episomal vector pLEXSY_IE-egfp-red-neo4
for inducible expression of fluorescent fusion proteins
EGE-1420neo 1 Kit 2.250,00    Add this product to your notepad  

Each kit contains:
  • pLEXSY_IE expression vector of choice
  • LEXSY host T7-TR as glycerol stocks
  • all components for preparation of 1 L LEXSY BHI medium
  • all selective antibiotics for 1 L medium
  • primer sets for insert sequencing
  • an easy-to-follow manual, see PDF file in "Downloads"

All components of the kit are available also separately.
Download the LEXSY vector map and sequence at:
We also provide all the molecular tools required for the construction of expression plasmids, such as

Please contact expression@jenabioscience.com with questions or inquiries.