Selected LEXSY References
 
Jřrgensen et al. (2014) Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae.
Microbial Cell Factories
13 doi: 10.1186/1475-2859-13-9.

 

Sepulveda et al. (2014) Expression, functionality, and localization of apurinic/apyrimidinic endonucleases in replicative and non-replicative forms of Trypanosoma cruzi. Journal of Cellular Biochemistry 115: 397
 

Zahedifard et al. (2014) Enhanced protective efficacy of nonpathogenic recombinant Leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. PLoS Neglected Tropical Diseases 8: e2751
 

Bhide et al. (2014) Rapid in vitro protein synthesis pipeline: a promising tool for cost-effective protein array design. Molecular BioSystems 10: 1236
 

Keller et al. (2014) Transduction of proteins into Leishmania tarentolae by formation of non-covalent complexes with cell-penetrating peptides. Journal of Cellular Biochemistry 115: 243
 

Keller et al. (2013) Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells. Pharmaceuticals 6: 184
 

Ruehrer and Michel (2013) Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers. Molecular Membrane Biology 30: 288
 

Fernandez,Vega (2013) Technologies to keep an eye on: alternative hosts for protein production in structural biology. Current Opinion in Structural Biology 23: 365
 

Klatt et al. (2013) Generation and characterization of a Leishmania tarentolae strain for site-directed in vivo biotinylation of recombinant proteins. Journal of Proteome Research 12: 5512
 

Klatt et al. (2013) Production of Glycosylated Soluble Amyloid Precursor Protein Alpha (sAPPalpha) in Leishmania tarentolae. Journal of Proteome Research 12: 396
 

Taromchi et al. (2013) Heterologous Expression of Human IL-29 (IFN-λ1) in Iranian Lizard Leishmania. Iranian Journal of Biotechnology 11: 168
 

Saljoughian et al. (2013) Development of novel prime-boost strategies based on a tri-gene fusion recombinant L. tarentolae vaccine against experimental murine visceral leishmaniasis. PLoS Neglected Tropical Diseases 7: e2174. doi: 10.1371/journal.pntd.0002174.

 

Hosseinzadeh et al. (2013) A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers. Drug Delivery 20: 190

 

Baechlein et al. (2013) Expression of a truncated hepatitis E virus capsid protein in the protozoan organism Leishmania tarentolae and its application in a serological assay. Journal of Virological Methods 193: 238
 

Salehi et al. (2012) Recombinant Leishmania tarentolae encoding the HPV type 16 E7 gene in tumor mice model. Immunotherapy 4: 1107. doi: 10.2217/imt.12.110.
 

Klatt et al. (2012) Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae. Microbial Cell Factories 11: 97
 

Hresko et al. (2012) Rapid protein production pipeline in advanced inducible Leishmania tarentolae expression system. In Rodrigues et al. (eds.) Farm animal proteomics part II: 75-79.
 

Dall & Brandstetter (2012) Activation of legumain involves proteolytic and conformational events, resulting in a context- and substrate-dependent activity profile. Acta Crystallographica F68: 24

 

Goodwin et al. (2012) Peptides as therapeutics with enhanced bioactivity. Current Medicinal Chemistry 19: 4451
 

Calvo-Álvarez et al. (2012)
Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis. PLoS Neglected Tropical Diseases;6(11):e1927.
 

Balańa-Fouce et al. (2012) Indotecan (LMP400) and AM13-55: Two novel indenoisoquinolines show potential for treating visceral leishmaniasis. Antimicrobial Agents and Chemotherapy doi: 10.1128/AAC.00499-12
 

Das et al. (2012) Vaccine development against Leishmania donovani. Frontiers in Immunology 3: doi: 10.3389/fimmu.2012.00099
 

Pirdel et al. (2012) Cloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System. Avicenna Journal of Medical Biotechnology 4: 186
 

Kumar et al. (2012) Overexpression of S4D Mutant of Leishmania donovani ADF/Cofilin Impairs Flagellum Assembly by Affecting Actin Dynamics. Eukaryotic cell 11: 752
 

Mishra et al. (2012) In Vitro Regulatory Effect of Epididymal Serpin CRES on Protease Activity of Proprotein Convertase PC4/PCSK4. Current Molecular Medicine 12: 1050
 

Hasebe et al. (2012) The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1. FEBS Letters 586: 16
 

Liu et al. (2012) Olfactomedin 4 Inhibits Cathepsin C-Mediated Protease Activities, Thereby Modulating Neutrophil Killing of Staphylococcus aureus and Escherichia coli in Mice. The Journal of Immunology 189: 2460
 

Niimi et al. (2012) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review. Methods in Molecular Biology 824: 307
 

Sugino et al. (2012) Expression of Multisubunit Proteins in Leishmania tarentolae. Methods in Molecular Biology, 824: 317
 

Dortay et al. (2011) High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection. PLoS ONE 6: 4 e18900
 

Dortay et al. (2011) Expression of human c-reactive protein in different systems and its purification from Leishmania tarentolae.
Protein Expression and Purification 78: 55
 

Kovtun et al. (2011) Leishmania cell-free protein expression System. Methods 55: 58
 

de La Llave et al. (2011) A combined luciferase imaging and reverse transcription polymerase chain reaction assay for the study of Leishmania amastigote burden and correlated mouse tissue transcript fluctuations. Cellular Microbiology 13: 81
 

Ghosh et al. (2011) Valeriana wallichii root extracts and fractions with activity against Leishmania spp. Parasitology Research 108: 861
 

McNamara et al. (2011) Target identification and validation of novel antimalarials Future Microbiology 6: 693
 

Mirzaahmadi et al. (2011) Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System. Journal of Biomedicine and Biotechnology doi:10.1155/2011/873874 Article ID 873874

 

Nazari et al. (2011) Cloning and expression of truncated form of tissue plasminogen activator in Leishmania tarentolae. Biotechnology Letters 33: 503
 

Davoudi et al. (2011) Cloning and expression of human IFN-gamma in Leishmania tarentolae. World Journal of Microbiology and Biotechnology 27: 1893
 

Dadashipour et al. (2011) Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems. Protein Expression and Purification doi:10.1016/j.pep.2010.12.010
 

Bolhassani et al. (2011) Fluorescent Leishmania species: Development of stable GFP expression and its application for in vitro and in vivo studies. Experimental Parasitology 127: 637
 

Sadlova et al. (2011) Visualisation of Leishmania donovani Fluorescent Hybrids during Early Stage Development in the Sand Fly Vector. PLoS ONE 6: 5 e19851
 

Kushnir et al. (2011) Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae. Molecular & Biochemical Parasitology 176: 69
 

Ben-Abdallah et al. (2011) Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae. Protein Expression and Purification 75: 114
 

Fernandez-Robledo et al. (2010) Production of recombinant proteins from protozoan parasites. Trends in Parasitology 26: 244 (Review)
 

Kovtun et al. (2010) Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System. PLOS one 5: e14388
 

Jacques et al. (2010) Functional characterization of LIT1, the Leishmania amazonensis ferrous iron transporter. Molecular & Biochemical Parasitology 170: 28
 

Hemayatkar et al. (2010) Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae. Biotechnology Journal 5: 1198
 

Gazdag et al. (2010) Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae. Acta Crystallographica F66: 871
 

Lecoeur et al. (2010) Early Curative Applications of the Aminoglycoside WR279396 on an Experimental Leishmania major-Loaded Cutaneous Site Do Not Impair the Acquisition of Immunity. Antimicrobial Agents and Chemotherapy 54: 984
 

Dortay et al. (2010) A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker. Microbial Cell Factories 9: 29
 

Smirlis et al. (2009) Leishmania donovani Ran-GTPase interacts at the nuclear rim with linker histone H1. Biochemical Journal 424: 367
 

Xingi et al. (2009) 6-Br-5methylindirubin-3'oxime (5-Me-6-BIO) targeting the leishmanial glycogen synthase kinase-3 (GSK-3) short form affects cell-cycle progression and induces apoptosis-like death: Exploitation of GSK-3 for treating leishmaniasis. International Journal for Parasitology 39: 1298
 

Heise et al. (2009) Molecular analysis of a UDP-GlcNAc:polypeptide α-N-acetylglucosaminyltransferase implicated in the initiation of mucin-type O-glycosylation in Trypanosoma cruzi. Glycobiology 19: 918
 

Bhattacharya et al. (2009) Role of a differentially expressed cAMP phosphodiesterase in regulating the induction of resistance against oxidative damage in Leishmania donovani. Free Radical Biology & Medicine 47: 1494
 

Foldynová-Trantírková et al. (2009) A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed
in Leishmania tarentolae. Journal of Biomolecular Structure & Dynamics 26: 755
 

Mureev et al. (2009) Species-independent translational leaders facilitate cell-free expression. Nature Biotechnology 27: 747
 

Phan et al. (2009) The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expression and Purification 68: 79
 

Basile et al. (2009) Recombinant protein expression in Leishmania tarentolae. Molecular Biotechnology 43: 273 (Review)
 

Basak et al. (2008) Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design. Protein Expression and Purification 60: 117
 

Fritsche et al. (2008) Development of a defined medium for heterologous expression in Leishmania tarentolae. Journal of Basic Microbiology 48: 488
 

Soleimani et al. (2007) Expression of human tissue-type plasminogen activator (t-PA) in Leishmania tarentolae. Biotechnology & Applied Biochemistry 48: 55
 

Lecoeur et al. (2007) Optimization of Topical Therapy for Leishmania major Localized Cutaneous Leishmaniasis Using a Reliable C57BL/6 Model PLoS Neglected Tropical Diseases 1: e34
 

Fritsche et al. (2007) Characterization of growth behaviour of Leishmania tarentolae – a new expression system for recombinant proteins. Journal of Basic Microbiology 47: 384
 

Mureev et al. (2007) Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers. Molecular & Biochemical Parasitology 155: 71
 

Niculae et al. (2006) Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae. Protein Expression and Purification 48: 167
 

Lukeš et al. (2006) Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context. Molecular & Biochemical Parasitology 148: 125
 

Kushnir et al. (2005) Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae. Protein Expression and Purification 42: 37
 

Lang et al. (2005) Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cellular Microbiology 7: 383
 

Barak et al. (2005) Differentiation of Leishmania donovani in host-free system: analysis of signal perception and response.
Molecular & Biochemical Parasitology 141: 99
 

Sodoyer (2004) Expression Systems for the Production of Recombinant Pharmaceuticals. Biodrugs 18: 51 (Review)
 

Breitling et al. (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expression and Purification 25: 209