Selected LEXSY References


Guo et al. (2016) A Single Glycan at the 99-Loop of Human Kallikrein-related Peptidase 2 Regulates Activation and Enzymatic Activity. Journal of Biological Chemistry 291:593.

Kianmehr et al. (2016) Cloning and expression of codon-optimized recombinant darbepoetin alfa in Leishmania tarentolae T7-TR. Protein Expression and Purification 118:120.

Fischer et al. (2016) Expression, characterisation and antigenicity of a truncated Hendra virus attachment protein expressed in the protozoan host Leishmania tarentolae. Journal of Virological Methods 228:48.


Rooney et al. (2015) Expression of Trypanosoma brucei gambiense Antigens in Leishmania tarentolae. Potential for Use in Rapid Serodiagnostic Tests (RDTs). PLOS Neglected Tropical Diseases DOI:10.1371/journal.pntd.0004271.

Bolhassani et al. (2015) VLP production in Leishmania tarentolae: A novel expression system for purification and assembly of HPV16 L1. Protein Expression and Purification 116:7.

Jones (2015) Leishmania tarentolae: an alternative approach to the production of monoclonal antibodies to treat emerging viral infections. Infectious Diseases of Poverty 4:8.

Katebi et al. (2015) Leishmania tarentolae secreting the sand fly salivary antigen PpSP15 confers protection against Leishmania major infection in a susceptible BALB/c mice model. Molecular Immunology 67:501.

Seif et al. (2015) EGFP reporter protein: its immunogenicity in Leishmania-infected BALB/c mice. Applied Microbiology and Biotechnology DOI 10.1007/s00253-015-7201-1.

Mlynarcik et al. (2015) Deciphering the interface between a CD40 receptor and borrelial ligand OspA. Microbiological Research 170:51.

Freier et al. (2015) Protease recognition sites in Bet v 1a are cryptic, explaining its slow processing relevant to its allergenicity. Scientific Reports 5:12707.

Gagorski et al. (2015) Performance benchmarking of four cell-free protein expression systems. Biotechnology and Bioengineering doi: 10.1002/bit.25814.

Buntu et al. (2015) A Versatile Coupled Cell-Free Transcription–Translation System Based on Tobacco BY-2 Cell Lysates. Biotechnology and Bioengineering, 112:867.

Stein et al. (2015) Towards the Systematic Mapping and Engineering of the Protein Prenylation Machinery in Saccharomyces cerevisiae. PLoS ONE 10:e0120716. doi:10.1371/journal.pone.0120716.

Bencurova et al. (2015) A rapid and simple pipeline for synthesis of mRNA–ribosome–VHH complexes used in single-domain antibody ribosome display. Molecular BioSystems 11:1515.

Kraeva et al. (2015) Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Genome Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani. PLOS Pathogens 11:e1005127.

Belaz et al. (2015) Identification, biochemical characterization, and in-vivo expression of the intracellular invertase BfrA from the pathogenic parasite Leishmania major. Carbohydrate Research 415:31.

Papadaki et al. (2015) The Leishmania donovani histidine acid ecto-phosphatase LdMAcP: insight into its structure and function. Biochemical Journal 467:473.

Kumar et al. (2015) HAT3-mediated acetylation of PCNA precedes PCNA monoubiquitination following exposure to UV radiation in Leishmania donovani. Nucleic Acids Research doi: 10.1093/nar/gkv431.


Shalev et al. (2015) Structural basis for selective targeting of leishmanial ribosomes: aminoglycoside derivatives as promising therapeutics. Nucleic Acids Research 34:8601.

Calvo-Álvarez et al. (2015) Infrared Fluorescent Imaging as a Potent Tool for In Vitro, Ex Vivo and In Vivo Models of Visceral Leishmaniasis. PLoS Neglected Tropical Diseases 9:e0003666. doi:10.1371/journal.pntd.0003666.


Taheri et al. (2015) Generation of stable L. major+EGFP-LUC and simultaneous comparison between EGFP and luciferase sensitivity. Experimental Parasitology 155:44.

Nasiri et al. (2015) Protective Efficacy of Live Leishmania tarentolae Expressing KMP11-NTGP96-GFP fusion as a vaccine candidate against visceral Leishmaniasis. Parasite Immunology PIM-2015-0045.R1

Yadegariet al. (2015) Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay. Iran J. Public Health 44:987.

García-Hernández et al. (2015) Fitness of Leishmania donovani Parasites Resistant to Drug Combinations. PLoS Neglected Tropical Diseases 9: e0003704.doi:10.1371/journal.pntd.0003704


Pion et al. (2014) Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae. Vaccine 32:5570.

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Guo et al. (2014) Sweetened kallikrein-related peptidases (KLKs): glycan trees as potential regulators of activation and activity. Biological Chemistry 395:959.

Chamakh-Ayari et al. (2014) In Vitro Evaluation of a Soluble Leishmania Promastigote Surface Antigen as a Potential Vaccine Candidate against Human Leishmaniasis PLOS ONE 9:e92708.

Dacher et al. (2014) Probing druggability and biological function of essential proteins in Leishmania combining facilitated null mutant and plasmid shuffle analyses. Molecular Microbiology 93:146.

Kraeva et al. (2014) Tetracycline-inducible gene expression system in Leishmania mexicana. Molecular and Biochemical Parasitology 198:11.

Rachidi et al. (2014) Pharmacological assessment defines Leishmania donovani casein kinase 1 as a drug target and reveals important functions in parasite viability and intracellular infection. Antimicrobial Agents and Chemotherapy 58:1501.

Seyed et al. (2014) Immunogenicity Evaluation of a Rationally Designed Polytope Construct Encoding HLA-A*0201 Restricted Epitopes Derived from Leishmania major Related Proteins in HLA-A2/DR1 Transgenic Mice: Steps toward
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Gonzalez-Leal et al. (2014) Cathepsin B in Antigen-Presenting Cells Controls Mediators of the Th1 Immune Response during Leishmania major Infection. PLOS Neglected Tropical Diseases 8:e3194.

Taheri et al. (2014) Expressional Comparison Between Episomal and Stable Transfection of a Selected tri-fusion Protein in Leishmania tarentolae. Vaccine Research 1:1.

Vacchina et al. (2014) In Vitro Screening Test Using Leishmania Promastigotes Stably Expressing mCherry Protein. Antimicrobial Agents and Chemotherapy 58:1825.

Chagas et al. (2014) Lundep, a sand fly salivary endonuclease increases Leishmania parasite survival in neutrophils and inhibits XIIa contact activation in human plasma. PLoS Pathogens 10:e1003923.


Goyard et al. (2014) In vivo imaging of trypanosomes for a better assessment of host-parasite relationships and drug efficacy. Parasitology International 63:260.

Jørgensen et al. (2014) Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae. Microbial Cell Factories 13 doi: 10.1186/1475-2859-13-9.

Han et al. (2014) Cortactin Scaffolds Arp2/3 and WAVE2 at the Epithelial Zonula Adherens. Journal of Biological Chemistry 289:7764.

Gambin et al. (2014) Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae eLife 3:e01434.

Brooks et al. (2014) Mechanism of Activation of Protein Kinase JAK2 by the Growth Hormone Receptor. Science 344:1249783.

Sepulveda et al. (2014) Expression, functionality, and localization of apurinic/apyrimidinic endonucleases in replicative and non-replicative forms of Trypanosoma cruzi. Journal of Cellular Biochemistry 115:397.

Zahedifard et al. (2014) Enhanced protective efficacy of nonpathogenic recombinant Leishmania tarentolae expressing cysteine proteinases combined with a sand fly salivary antigen. PLoS Pathogens 8: e2751

Bhide et al. (2014) Rapid in vitro protein synthesis pipeline: a promising tool for cost-effective protein array design. Molecular BioSystems 10:1236.

Keller et al. (2014) Transduction of proteins into Leishmania tarentolae by formation of non-covalent complexes with cell-penetrating peptides. Journal of Cellular Biochemistry 115:243.


Keller et al. (2013) Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells. Pharmaceuticals 6:184.

Guo et al. (2013) Subunit Organisation of In Vitro Reconstituted HOPS and CORVET Multisubunit Membrane Tethering Complexes. PLoS ONE 8:e81534.

Ruehrer and Michel (2013) Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers. Molecular Membrane Biology 30:288.

Fernandez,Vega (2013) Technologies to keep an eye on: alternative hosts for protein production in structural biology. Current Opinion in Structural Biology 23:365.

Klatt et al. (2013) Generation and characterization of a Leishmania tarentolae strain for site-directed in vivo biotinylation of recombinant proteins. Journal of Proteome Research 12:5512.

Klatt et al. (2013) Production of Glycosylated Soluble Amyloid Precursor Protein Alpha (sAPPalpha) in Leishmania tarentolae. Journal of Proteome Research 12:396.

Taromchi et al. (2013) Heterologous Expression of Human IL-29 (IFN-λ1) in Iranian Lizard Leishmania. Iranian Journal of Biotechnology 11:168.

Saljoughian et al. (2013) Development of novel prime-boost strategies based on a tri-gene fusion recombinant L. tarentolae vaccine against experimental murine visceral leishmaniasis. PLoS Neglected Tropical Diseases 7:e2174. doi: 10.1371/journal.pntd.0002174.


Hosseinzadeh et al. (2013) A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers. Drug Delivery 20:190.

Baechlein et al. (2013) Expression of a truncated hepatitis E virus capsid protein in the protozoan organism Leishmania tarentolae and its application in a serological assay. Journal of Virological Methods 193:238.


Salehi et al. (2012) Recombinant Leishmania tarentolae encoding the HPV type 16 E7 gene in tumor mice model. Immunotherapy 4:1107. doi: 10.2217/imt.12.110.

Klatt et al. (2012) Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae. Microbial Cell Factories 11:97.

Hresko et al. (2012) Rapid protein production pipeline in advanced inducible Leishmania tarentolae expression system. In Rodrigues et al. (eds.) Farm animal proteomics part II:75-79.

Dall & Brandstetter (2012) Activation of legumain involves proteolytic and conformational events, resulting in a context- and substrate-dependent activity profile. Acta Crystallographica F68:24.

Goodwin et al. (2012) Peptides as therapeutics with enhanced bioactivity. Current Medicinal Chemistry 19:4451.

Calvo-Álvarez et al. (2012) Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis. PLoS Neglected Tropical Diseases 6(11):e1927.

Balaña-Fouce et al. (2012) Indotecan (LMP400) and AM13-55: Two novel indenoisoquinolines show potential for treating visceral leishmaniasis. Antimicrobial Agents and Chemotherapy doi: 10.1128/AAC.00499-12

Das et al. (2012) Vaccine development against Leishmania donovani. Frontiers in Immunology 3: doi: 10.3389/fimmu.2012.00099

Pirdel et al. (2012) Cloning and Expression of Leishmania infantum LPG3 Gene by the Lizard Leishmania Expression System. Avicenna Journal of Medical Biotechnology 4:186.

Kumar et al. (2012) Overexpression of S4D Mutant of Leishmania donovani ADF/Cofilin Impairs Flagellum Assembly by Affecting Actin Dynamics. Eukaryotic cell 11:752.

Mishra et al. (2012) In Vitro Regulatory Effect of Epididymal Serpin CRES on Protease Activity of Proprotein Convertase PC4/PCSK4. Current Molecular Medicine 12:1050.

Hasebe et al. (2012) The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1. FEBS Letters 586:16.

Liu et al. (2012) Olfactomedin 4 Inhibits Cathepsin C-Mediated Protease Activities, Thereby Modulating Neutrophil Killing of Staphylococcus aureus and Escherichia coli in Mice. The Journal of Immunology 189:2460.

Niimi et al. (2012) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review. Methods in Molecular Biology 824:307.

Sugino et al. (2012) Expression of Multisubunit Proteins in Leishmania tarentolae. Methods in Molecular Biology 824:317.


Dortay et al. (2011) High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection. PLoS ONE 6:4 e18900.

Dortay et al. (2011) Expression of human c-reactive protein in different systems and its purification from Leishmania tarentolae.
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Kovtun et al. (2011) Leishmania cell-free protein expression System. Methods 55:58.

de La Llave et al. (2011) A combined luciferase imaging and reverse transcription polymerase chain reaction assay for the study of Leishmania amastigote burden and correlated mouse tissue transcript fluctuations. Cellular Microbiology 13:81.

Ghosh et al. (2011) Valeriana wallichii root extracts and fractions with activity against Leishmania spp. Parasitology Research 108:861.

McNamara et al. (2011) Target identification and validation of novel antimalarials Future Microbiology 6:693.

Mirzaahmadi et al. (2011) Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System. Journal of Biomedicine and Biotechnology doi:10.1155/2011/873874 Article ID 873874.


Nazari et al. (2011) Cloning and expression of truncated form of tissue plasminogen activator in Leishmania tarentolae. Biotechnology Letters 33:503.

Davoudi et al. (2011) Cloning and expression of human IFN-gamma in Leishmania tarentolae. World Journal of Microbiology and Biotechnology 27:1893.

Dadashipour et al. (2011) Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems. Protein Expression and Purification doi:10.1016/j.pep.2010.12.010

Bolhassani et al. (2011) Fluorescent Leishmania species: Development of stable GFP expression and its application for in vitro and in vivo studies. Experimental Parasitology 127:637.

Sadlova et al. (2011) Visualisation of Leishmania donovani Fluorescent Hybrids during Early Stage Development in the Sand Fly Vector. PLoS ONE 6:5 e19851.

Kushnir et al. (2011) Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae. Molecular & Biochemical Parasitology 176:69.

Ben-Abdallah et al. (2011) Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae. Protein Expression and Purification 75:114.


Fernandez-Robledo et al. (2010) Production of recombinant proteins from protozoan parasites. Trends in Parasitology 26:244. (Review)

Kovtun et al. (2010) Towards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System. PLOS one 5:e14388.

Jacques et al. (2010) Functional characterization of LIT1, the Leishmania amazonensis ferrous iron transporter. Molecular & Biochemical Parasitology 170:28.

Hemayatkar et al. (2010) Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae. Biotechnology Journal 5:1198.

Gazdag et al. (2010) Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae. Acta Crystallographica F66:871.

Lecoeur et al. (2010) Early Curative Applications of the Aminoglycoside WR279396 on an Experimental Leishmania major-Loaded Cutaneous Site Do Not Impair the Acquisition of Immunity. Antimicrobial Agents and Chemotherapy 54:984.

Dortay et al. (2010) A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker. Microbial Cell Factories 9:29.


Smirlis et al. (2009) Leishmania donovani Ran-GTPase interacts at the nuclear rim with linker histone H1. Biochemical Journal 424:367.

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Phan et al. (2009) The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expression and Purification 68:79.

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Basak et al. (2008) Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design. Protein Expression and Purification 60:117.

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Niculae et al. (2006) Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae. Protein Expression and Purification 48:167.

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Kushnir et al. (2005) Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae. Protein Expression and Purification 42:37.

Lang et al. (2005) Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cellular Microbiology 7:383.

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Breitling et al. (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Protein Expression and Purification 25:209.