Enzyme Assays - GTPase Activation Kits
GTPase Activation Kits provide rapid, cost-effective and reliable tools for the detection and semi-quantitative analysis of the cellular activation state of several GTPases.
Active GTPase (i.e. complexed to GTP), is purified from cell extracts via a one-step batch affinity purification step exploiting its affinity towards effector proteins that selectively interact with the active, GTP-bound GTPase conformation, which is consequently detected by Western blotting applying the included antibody.
The kits also include the appropriate recombinant GTPase proteins that, once preloaded with GDP or GTPγS (or GppNHp, respectively), can be used to "spike" cell extracts, thus serving as an internal control of signal specificity.
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