Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody binding domains. These high affinity regions are specifically bound to the Fc region of immunoglobulins (IgGs).

Protein A is temperature stable and it retains its native conformation even in the presence of denaturing agents. Protein A resins have been widely used to purify a broad range of immunoglobulins from different mammalian species.

   Protein A and G affinity towards various species and IgG isotypes

High IgG-binding capacity Milder elution condition than Protein G resin Highly stable bound Protein A: resin is reusable with no significant loss of binding capacity

Protein A Agarose characteristics Matrix 4% cross-linked agarose Bead size 40-180 µm Binding capacity ~ 25 mg human IgG / ml resin Ligand density ~ 3 mg rProtein A / ml resin Linear flow rate 26 cm/h Maximum pressure 0.18 bar (2.6 psi)

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