Catalog Nickel, Zinc, Cobalt, Copper and Metal Free IDA Agaroses, Gravity Flow - Nickel AgarosesNickel, Zinc, Cobalt, Copper and Metal Free IDA Agaroses, Gravity Flow - Nickel Agaroses
 
 
Nickel Agaroses   |   Zinc Agaroses   |   Cobalt and Copper Agaroses   |   Metal Free Agaroses
 
Suitable for gravity flow and batch purification

His-tagged proteins are efficiently purified by a one-step procedure from crude lysates both under denaturing and non-denaturing conditions.
 


  • Tridentate IDA-linker for protein elution with lower imidazole concentrations compared to tetradentate chelators (e.g. NTA)
  • Precharged material with four metal types (Cu2+, Ni2+, Zn2+, Co2+) as well as metal free agarose
  • Two metal loading densities
  • Different formats: bulk material, pre-packed columns and spin columns for standard microcentrifuge

Product characteristics

Matrix6 % cross-linked agarose
Bead size50-150 µm
LinkerIminodiacetic acid (IDA)
Metal loading capacityLow density: 5-20 µmol Me2+/ ml resin
High density: 20-40 µmol Me2+/ ml resin
Linear flow rate26 cm/h
Maximum pressure0.18 bar (2.6 psi)
pH stability2-12
Chemical stabilityStable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride

 

Optimize your purification strategy by a flexible choice of metal ion and metal loading density

Affinity Chromatography - HIs-tagged Proteins Density vs. Specificity
Based on the HSAP concept, IDA-immobilized Cu2+, Ni2+, Zn2+ and Co2+ ions exhibit different affinities & specificities towards histidines [2,3]. Copper (Cu2+): Shows the lowest specificity resulting in high target recoveris. Unspecific protein binding is minimized by low density metal loading. Cobalt (Co2+): Shows the highest binding specificity resulting in reduced unspecific protein binding. Target loss is minimized by high density metal loading. Nickel (Ni2+) and Zinc (Zn2+): Show intermediate selectivity. While using Ni2+ ions is the standard method, IDA-immobilized Zn2+ may prove superior to either immobilized Cu2+ and Ni2+ ions, as a result of its relatively low binding affinity for E. coli host cell proteins[4]. Low density metal loading enhances the qualitative purification of recombinant protein but with lower target recoveries. High density metal loading results in greater purification of recombinant proteins. However, unwanted proteins within the sample will also be bound.

 

High Density Nickel Agarose    
Product Cat. No. Amount Price (EUR) Buy / Note  
Bulk material - 25 ml AC-303-25 25 ml 145,00    Add this product to your notepad  
Bulk material - 100 ml AC-303-100 100 ml 400,00    Add this product to your notepad
Bulk material - 500 ml AC-303-500 500 ml 1.800,00    Add this product to your notepad

 
Low Density Nickel Agarose    
Product Cat. No. Amount Price (EUR) Buy / Note  
Bulk material - 25 ml AC-304-25 25 ml 145,00    Add this product to your notepad  
Bulk material - 100 ml AC-304-100 100 ml 400,00    Add this product to your notepad
Bulk material - 500 ml AC-304-500 500 ml 1.800,00    Add this product to your notepad

 
Selected references:

[1] Porath et al. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598.

[2] Gaberc-Porekar et al. (2001) Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 49:335.

[3] Ueda et al. (2003) Current and prospective applications of metal ion–protein binding. Journal of Chromatography 988:1.

[4] Richard et al. (2000) Design of Affinity Tags for One-Step Protein Purification from Immobilized Zinc Columns. Biotechnol. Prog. 16:86.
 

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