Immobilized phospho-aminoacids provide a convenient and rapid purification procedure for proteins that bind to particular phosphorylated amino acids [1-3].

Jena Bioscience offers affinity chromatography material that is taylor-made for purification (or for related assays) of particular phospho-aminoacid binding proteins since we offer different types and lengths of linkers and provide different types of chromatography material ranging from bulk media to pre-packed columns that fit any machine.

Immobilized O-Phospho-Serine

Immobilized O-Phospho-Threonine

Immobilized O-Phospho-Tyrosine

Unmodified Agarose

Selected References:

[1] Bakalara et al. (2000) Purification, Cloning, and Characterization of an Acidic Ectoprotein Phosphatase differentially expressed in the infectious bloodstream form of trypanosoma brucei. J. Biol. Chem. 275 (12):8863.

[2] Muir et al. (1998) Expressed protein ligation: A general method for protein engineering. Proc. Natl. Acad. Sci. USA 95:6705.

[3] Pei et al. (1996) Phospholipase C-y1 binds to actin-cytoskeleton via its C-terminal SH2 domain in vitro. Biochem. Biophys. Res. Comm. 228:802.

Different types of chromatography material available

All our affinity chromatography materials carrying nucleotides or amino/phospho-amino acids are available as bulk media, pre-packed MPLC columns, syringe columns and screening columns. Please refer to the product pages to access the product lists and data sheets, and to order affinity chromatography products.

Bulk media	Pre-packed syringe columns	Pre-packed screening columns	Pre-packed MPLC columns
based on Agarose (equals Sepharose™ 4B)	based on Agarose (equals Sepharose™ 4B)	based on Agarose (equals Sepharose™ 4B) maximum amount 0.6 ml ready-to-use glass columns one each upper and lower frit upper and lower end piece special 96-well-MTP format column holder on request	based on Toyopearl® AF-650M 40 - 90 µ material 1x Omnifit column kit 10x50 mm 1x adjustable end piece 2x female-female (1/4" to 6 mm) coupling 2 m PTFE tubing (1/16" OD x 0.8 mm ID) MPLC columns are delivered with adapters for FPLC Systems of BioRad, GE Healthcare (Äkta™) and Pharmacia.

Toyopearl^® is a trademark of TOSOH Bioscience
Äkta™ and Sepharose™ are trademarks of GE Healthcare Companies

Please contact us at info@jenabioscience.com for more information.