Catalog Real-Time PCR - qPCR Core KitsReal-Time PCR - qPCR Core Kits
 
 
qPCR Mixes for Dual Labeled Fluorescent Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 
qPCR Core Kit
Complete sets of reagents required for Real-Time PCR

qPCR Core Kits are designed for the quantitative real-time analysis of DNA samples. The kits provide powerful tools for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision.
The high specificity and sensitivity of the Core Kits is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The included dNTP mix contains dUTP instead of dTTP and allows an UNG (Uracil-N-Glycosylase) treatment at the onset of thermal cycling to prevent carry-over contaminations of DNA from previous PCR reactions.
 

qPCR Probes Core Kit    
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-331S 100 reactions x 50 Ál 90,00    Add this product to your notepad  
L pack PCR-331L 500 reactions x 50 Ál 360,00    Add this product to your notepad

Store at -20░C, avoid frequent thawing and freezing

qPCR Pol
10x qPCR ProbesBuffer complete
10x qPCR ProbesBuffer without MgCl2
MgCl2 stock solution
dNTP Mix incl. dUTP
qPCR Probes Core Kit is optimized for block-based PCR instruments. The high specificity and sensitivity of the kit is achieved by an optimized hot-start polymerase. Its thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
 
qPCR Green Core Kit    
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-333S 100 reactions x 50 Ál 90,00    Add this product to your notepad  
L pack PCR-333L 500 reactions x 50 Ál 360,00    Add this product to your notepad

Store at -20░C, avoid frequent thawing and freezing
qPCR buffer +ROX must be stored in the dark

qPCR Pol
10x qPCR GreenBuffer complete
10x qPCR GreenBuffer without MgCl2
MgCl2 stock solution
dNTP Mix incl. dUTP
qPCR Green Core Kit contains EvaGreen fluorescent DNA stain. EvaGreen is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications and a superior replacement for the widely used SYBR« Green I dye.
 

EvaGreen« is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR« is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.