. . qPCR Mixes with EvaGreen, Real-Time PCR, Molecular Biology, Products & Ordering, Jena Bioscience
 
 
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qPCR Mixes for Dual Labeled Fluorescent Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 
qPCR Master Mixes
qPCR Mixes with EvaGreen™

JBS qPCR GreenMaster Series allows quantitative real-time analysis of DNA samples based on EvaGreen™ fluorescent DNA stain. During PCR, EvaGreen™ intercalates into the amplified DNA. No synthesis of labeled probes is required, thus target DNA may rapidly be analyzed. Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated solution, including hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of initial denaturation to prevent extension of nonspecifically annealed primers and primer-dimer formation.
Both qPCR GreenMaster mixes with and without UNG are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization. To facilitate pipetting into opaque plasticware a blue dyed version of the qPCR ProbesMaster Mixes is available.

EvaGreen™ Fluorescent DNA Stain
EvaGreen™ Fluorescent DNA Stain is developed for DNA analysis including real-time PCR (qPCR), high-resolution DNA melting curve analysis (HRM), routine DNA quantification and capillary gel electrophoresis. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically.


 

qPCR GreenMaster with UNG
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-303S 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-303L 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-303S-bl 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-303L-bl 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with UNG must be stored in the dark

qPCR GreenMaster with UNG, 2x conc.
PCR-grade water
qPCR GreenMaster with UNG contains UNG (Uracil-N-Glycosylase).
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The reaction chemistry of the kit is optimized for block-based PCR instruments.
 
qPCR GreenMaster
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-313S 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-313L 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-313S-bl 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-313L-bl 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster must be stored in the dark

qPCR GreenMaster, 2x conc.
PCR-grade water
qPCR GreenMaster is optimized for block-based PCR instruments.
 
qPCR GreenMaster with UNG/lowROX
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-306S 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-306L 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-306S-bl 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-306L-bl 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with UNG/lowROX must be stored in the dark

qPCR GreenMaster with UNG/lowROX, 2x conc.
PCR-grade water
qPCR GreenMaster with UNG/lowROX contains UNG (Uracil-N-Glycosylase) and 50 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR GreenMaster with lowROX
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-316S 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-316L 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-316S-bl 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-316L-bl 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with lowROX must be stored in the dark

qPCR GreenMaster with lowROX, 2x conc.
PCR-grade water
qPCR GreenMaster with lowROX contains 50 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR GreenMaster with UNG/highROX
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-304S 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-304L 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-304S-bl 2 x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-304L-bl 10 x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with UNG/highROX must be stored in the dark

qPCR GreenMaster with UNG/highROX, 2x conc.
PCR-grade water
qPCR GreenMaster with UNG/highROX contains UNG (Uracil-N-Glycosylase) and 500 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR GreenMaster with highROX
Master mix for real-time qPCR with green-fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-314S 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-314L 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-314S-bl 2 x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-314L-bl 10 x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
qPCR GreenMaster with highROX must be stored in the dark

qPCR GreenMaster with highROX, 2x conc.
PCR-grade water
qPCR GreenMaster with highROX contains 500 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 

EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.