Catalog Real-Time PCR - qPCR Mixes for Dual Labeled Fluorescent ProbesReal-Time PCR - qPCR Mixes for Dual Labeled Fluorescent Probes
 
 
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qPCR Mixes for Dual Labeled Fluorescent Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 
qPCR Master with ROX
qPCR Ready-to-Use Mixes for Dual Labeled Fluorescent Probes ensure
highest quality of amplification results


The JBS qPCR Master Series is designed for the quantitative real-time analysis of DNA samples using DNA probe based detection. The mixes are recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan, Molecular Beacons or FRET probes. They provide easy-to-handle and powerful tools for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision.
The Master Mixes contain all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mixes is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.

qPCR ProbesMaster mixes with or without UNG are available in combination with low ROX and high ROX as reference dye for cycler-internal signal normalization.



The blue dyed versions of the qPCR ProbesMaster mixes contain an inert blue dye for easier handling. They are specially recommended to facilitate pipetting in combination with white or opaque plasticware that guarantees an optimized fluorescence signal recovery.
 

qPCR ProbesMaster with UNG
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-301S 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-301L 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-301S-bl 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-301L-bl 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible

qPCR ProbesMaster with UNG, 2x conc.
PCR-grade water
qPCR ProbesMaster with UNG contains UNG (Uracil-N-Glycosylase).
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The reaction chemistry of the kit is optimized for block-based PCR instruments.
 
qPCR ProbesMaster
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-311S 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-311L 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-311S-bl 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-311L-bl 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible

qPCR ProbesMaster, 2x conc.
PCR-grade water
qPCR ProbesMaster is optimized for block-based PCR instruments.
 
qPCR ProbesMaster with UNG/lowROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-305S 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-305L 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-305S-bl 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-305L-bl 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with UNG/lowROX must be stored in the dark

qPCR ProbesMaster with UNG/lowROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with UNG/lowROX contains UNG (Uracil-N-Glycosylase) and 50 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with lowROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-315S 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-315L 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-315S-bl 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-315L-bl 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with lowROX must be stored in the dark

qPCR ProbesMaster with lowROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with lowROX contains 50 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with UNG/highROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-302S 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad  
clear - L pack PCR-302L 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad
blue dyed - S pack PCR-302S-bl 2x 1,25 ml
(2x conc.)
130,00    Add this product to your notepad
blue dyed - L pack PCR-302L-bl 10x 1,25 ml
(2x conc.)
520,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with UNG/highROX must be stored in the dark

qPCR ProbesMaster with UNG/highROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with UNG/highROX contains UNG (Uracil-N-Glycosylase) and 500 nM ROX passive reference dye in the final assay.
UNG and dUTP instead of dTTP is used to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
The ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.
 
qPCR ProbesMaster with highROX
Master mix for quantitative real-time PCR using labeled DNA probes
   
Product Cat. No. Amount Price (EUR) Buy / Note  
clear - S pack PCR-312S 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad  
clear - L pack PCR-312L 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad
blue dyed - S pack PCR-312S-bl 2x 1,25 ml
(2x conc.)
110,00    Add this product to your notepad
blue dyed - L pack PCR-312L-bl 10x 1,25 ml
(2x conc.)
440,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing
Storage at 4C for up to 3 months possible
qPCR ProbesMaster with highROX must be stored in the dark

qPCR ProbesMaster with highROX, 2x conc.
PCR-grade water
qPCR ProbesMaster with highROX contains 500 nM ROX passive reference dye in the final assay.
The ROX dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The reaction chemistry of the kit is optimized for block-based PCR instruments that are compatible with the evaluation of the ROX reference signal.