Catalog DNA Sequencing (Sanger Method) - Dideoxynucleotides (ddNTPs)DNA Sequencing (Sanger Method) - Dideoxynucleotides (ddNTPs)
 
 
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Sequencing Kits   |   Sequencing Polymerases   |   Dideoxynucleotides (ddNTPs)   |   Termination Mixes
 
Dideoxynucleotide triphosphates (ddNTPs) lack the 3'-OH group of dNTPs that is essential for polymerase-mediated strand elongation in a PCR. Therefore, ddNTPs are used in combination with a modified Taq polymerase (e.g. JBS Sequencing polymerase or Thermosequenase™) as 3'-end chain terminators in Sanger sequencing[1] (Fig. 1) and single nucleotide polymorphisms (SNPs) genotyping by single base pair extension (SBE)[2] (Fig. 2). Our ddNTPs are > 98 % pure (HPLC) and functionally tested in chain termination sequencing.
 
Sequencing PolymerasedNTPs5'-fluorescently labeled PrimerSanger Sequencing
 
Figure 1: The enzymatic dideoxy chain termination sequencing method of Sanger[1] relies on the linear amplification of a single-stranded template DNA using a 5'-fluorescently labeled primer and a modified Taq polymerase. The synthesis of the complementary DNA strand starts at the specific priming site and ends with the incorporation of a chain-terminating ddNTP that is randomly introduced instead of its corresponding dNTP. By using the four different ddNTPs in four separate reaction vials, a set of extended primer strands terminated at each A, C, G, and T are obtained. When these fragments are separated on a suitable gel matrix the sequence information can be obtained from the migration order of the bands (bottom to top).
 
Make sure you also check out our DNA Cycle Sequencing Kit!
 
Sequencing PolymerasePrimerSBE
 
Figure 2: The single basepair extension (SBE) assay is a reliable method for the detection of a priori known locations of single nucleotide polymorphisms (SNPs)[2]. It relies on the extension of a primer, designed to bind one nucleotide upstream of the polymorphic spot, by a matching ddNTP. Subsequent detection of the incorporated ddNTP is performed by mass spectrometry of the extended primer, thus revealing the nucleotide base at that position on the template strand[3].
 

ddNTP bundle
4x 10 mM (ddATP, ddCTP, ddGTP, ddTTP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1019S 4 x 100 Ál
(4 x 1 Ámol)
117,67    Add this product to your notepad  
L pack NU-1019L 4 x 500 Ál
(4 x 5 Ámol)
529,52    Add this product to your notepad

Store at -20░C, short term (up to one week) exposure
to ambient temperature possible
ddNTP Bundle contains four separate solutions of ultrapure, sequencing grade ddATP, ddCTP, ddGTP, and ddTTP.
 
ddATP
10 mM
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1015S 100 Ál
(1 Ámol)
36,75    Add this product to your notepad  
L pack NU-1015L 5 x 100 Ál
(5 Ámol)
165,33    Add this product to your notepad

Store at -20░C, short term (up to one week) exposure
to ambient temperature possible
Aqueous solution of ultrapure, sequencing grade ddATP.
 
ddCTP
10 mM
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1016S 100 Ál
(1 Ámol)
36,75    Add this product to your notepad  
L pack NU-1016L 5 x 100 Ál
(5 Ámol)
165,33    Add this product to your notepad

Store at -20░C, short term (up to one week) exposure
to ambient temperature possible
Aqueous solution of ultrapure, sequencing grade ddCTP.
 
ddGTP
10 mM
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1017S 100 Ál
(1 Ámol)
36,75    Add this product to your notepad  
L pack NU-1017L 5 x 100 Ál
(5 Ámol)
165,33    Add this product to your notepad

Store at -20░C, short term (up to one week) exposure
to ambient temperature possible
Aqueous solution of ultrapure, sequencing grade ddGTP.
 
ddTTP
10 mM
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1018S 100 Ál
(1 Ámol)
36,75    Add this product to your notepad  
L pack NU-1018L 5 x 100 Ál
(5 Ámol)
165,33    Add this product to your notepad

Store at -20░C, short term (up to one week) exposure
to ambient temperature possible
Aqueous solution of ultrapure, sequencing grade ddTTP.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com