Catalog Alkyne Reagents - Auxiliary ReagentsAlkyne Reagents - Auxiliary Reagents
 
 
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The efficiency of a copper (Cu(I))-catalyzed Azide-Alkyne click chemistry reaction (CuAAC) strongly depends on the presence of copper ions in the +1 oxidation state (Cu(I)).

Different copper catalyst sources, reduction reagents and Cu(I) stabilizing ligands are available (Tab. 1) however, for most bioconjugation applications the combination of CuSO4 as copper catalyst source, sodium ascorbate as a reduction reagent and THPTA as Cu(I) stabilizing ligand is recommended.

Presolski et. al.[1] (Download pdf) and Hong et. al.[2] (Download pdf & supplemental information) provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
 


Table 1: Overview of available reaction reagents for Cu(I) catalyzed Azide-Alkyne click chemistry (CuAAC).

Copper (Cu) catalystReduction reagentCu(I) stabilizing Ligand
CuSO4Sodium AscorbateTHPTA
CuBrTBTA
 

Protein Reaction Buffer Kit    
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit CLK-1001 1 Kit 149,86    Add this product to your notepad  

 
Click Solvent
(DMSO / tert-Butanol, 3:1)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
1 ml pack CLK-MI003-1 1 ml 12,39    Add this product to your notepad  
10x 1 ml pack CLK-MI003-10 10 x 1 ml 94,76    Add this product to your notepad

 
CuSO4 - click chemistry grade
(Copper(II)-Sulphate)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-MI004-10 10 mg 12,70    Add this product to your notepad  
5x 10 mg pack CLK-MI004-50 5 x 10 mg 47,23    Add this product to your notepad

 
CuBr - click chemistry grade
(Copper(I)-Bromide)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
5 mg pack CLK-MI001-5 5 mg 12,70    Add this product to your notepad  
50 mg pack CLK-MI001-50 50 mg 104,80    Add this product to your notepad

 
Na-Ascorbate    
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-MI005-10 10 mg 13,34    Add this product to your notepad  
5x 10 mg pack CLK-MI005-50 5 x 10 mg 50,18    Add this product to your notepad

 
THPTA
Tris(3-hydroxypropyltriazolylmethyl)amine
   
Product Cat. No. Amount Price (EUR) Buy / Note  
100 mg pack CLK-1010-100 100 mg 133,61    Add this product to your notepad  
1 g pack CLK-1010-1G 1 g 404,88    Add this product to your notepad

 
TBTA-Ligand
(Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
5 mg pack CLK-MI002-5 5 mg 30,59    Add this product to your notepad  
10 mg pack CLK-MI002-10 10 mg 51,66    Add this product to your notepad

 

Selected references:

[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
 
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