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LEXSY Configurations - Vectors and PrimersInducible (Genome integrated)
| Inducible (Episomal)
| Constitutive (Genome integrated)
| Vectors and Primers
| In Vitro (cell-free)
| Custom Expression
The constitutive pLEXSY-2 vectors (EGE-231 to EGE-234) and inducible pLEXSY_I vectors (EGE-243 to EGE-251) were developed for both, intracellular or secretory expression of target proteins.
For constitutive expression we recommend the standard vector pLEXSY-sat2 for selection of LEXSY expression strains with the antibiotic LEXSY NTC. For inducible expression we recommend the integrative vector pLEXSY_I-blecherry3 for selection of LEXSY expression strains with the antibiotic LEXSY Bleo and Cherry fluorescence monitoring of induction.
For expression plasmid construction we recommend our ligase-free CyClone technology in combination with our Primer Designer software (web shop account required)!
| LEXSY Expression Vectors | |||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| pLEXSY-ble2 integrative constitutive expression vector antibiotic selection of transfectants with LEXSY Bleo |
EGE-231 | 5 µg | 480,00 | 
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| pLEXSY-hyg2 integrative constitutive expression vector antibiotic selection of transfectants with LEXSY Hygro |
EGE-232 | 5 µg | 480,00 |
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| pLEXSY-neo2 integrative constitutive expression vector antibiotic selection of transfectants with LEXSY Neo |
EGE-233 | 5 µg | 480,00 |
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| pLEXSY-sat2 integrative constitutive expression vector antibiotic selection of transfectants with LEXSY NTC |
EGE-234 | 5 µg | 480,00 |
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| pLEXSY_I-blecherry3 integrative inducible expression vector antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with Cherry fluorescence |
EGE-243 | 5 µg | 480,00 |
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| pLEXSY_I-ble3 integrative inducible expression vector antibiotic selection of transfectants with LEXSY Bleo |
EGE-244 | 5 µg | 480,00 |
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| pLEXSY_I-neo3 integrative inducible expression vector antibiotic selection of transfectants with LEXSY Neo |
EGE-245 | 5 µg | 480,00 |
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| pLEXSY_IE-blecherry4 episomal inducible expression vector antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with Cherry fluorescence |
EGE-255 | 5 µg | 480,00 |
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The LEXSY Control Vectors were developed as a positive transfection control. They allow evaluation of expression by simple fluorescence measurement of suspension cultures. As reporter proteins the Green, Red, or Cherry Fluorescence Proteins are available.
| LEXSY Control Vectors | |||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| pLEXSY-egfp-sat2 integrative constitutive control vector with egfp gene antibiotic selection of transfectants with LEXSY NTC and expression monitoring with EGFP fluorescence |
EGE-235 | 5 µg | 480,00 |
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| pLEXSY-cherry-sat2 integrative constitutive control vector with cherry gene antibiotic selection of transfectants with LEXSY NTC and expression monitoring with Cherry fluorescence |
EGE-236 | 5 µg | 480,00 |
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| pLEXSY-red-sat2 integrative constitutive control vector with Ds gene antibiotic selection of transfectants with LEXSY NTC and expression monitoring with Ds red fluorescence |
EGE-237 | 5 µg | 480,00 |
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| pLEXSY_I-egfp-blecherry3 integrative inducible control vector with egfp gene antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with EGFP and Cherry fluorescence |
EGE-246 | 5 µg | 480,00 |
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| pLEXSY_I-egfp-ble3 integrative inducible control vector with egfp gene antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with EGFP fluorescence |
EGE-247 | 5 µg | 480,00 |
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| pLEXSY_I-egfp-neo3 integrative inducible control vector with egfp gene antibiotic selection of transfectants with LEXSY Neo and expression monitoring with EGFP fluorescence |
EGE-248 | 5 µg | 480,00 |
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The LEXSY Primers were designed for insert sequencing and diagnostic PCR of recombinant LEXSY strains based on the LEXSY expression vectors.
| Primers | |||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| A264 insert sequencing reverse primer (for all expression vectors) |
PM-101 | 2,5 nmol | 22,00 |
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| A992 insert sequencing forward primer (for all "F4" expression vectors with 5'utr camBA) |
PM-102 | 2,5 nmol | 22,00 |
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| F2999 sat forward primer (for integration diagnostics of all sat expression vectors) |
PM-103 | 2,5 nmol | 22,00 |
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| F3002 ssu reverse primer (for integration diagnostics of all ssu integration vectors) |
PM-104 | 2,5 nmol | 22,00 |
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| F3001 ssu forward primer (for integration diagnostics of all ssu integration vectors) |
PM-105 | 2,5 nmol | 22,00 |
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| A1045 F4 reverse primer (for integration diagnostics of all "F4" expression vectors with 5'utr camBA) |
PM-106 | 2,5 nmol | 22,00 |
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| A708 ble forward primer (for integration diagnostics of all ble expression vectors) |
PM-107 | 2,5 nmol | 22,00 |
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| A1432 neo forward primer (for integration diagnostics of all neo expression vectors) |
PM-108 | 2,5 nmol | 22,00 |
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| A3804 hyg forward primer (for integration diagnostics of all hyg expression vectors) |
PM-109 | 2,5 nmol | 22,00 |
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| P1442 Insert sequencing forward primer (for all "AP" expression vectors with 5'utr aprt) |
PM-110 | 2,5 nmol | 22,00 |
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| A1715 AP reverse primer (for integration diagnostics of all "AP" expression vectors with 5'utr aprt) |
PM-111 | 2,5 nmol | 22,00 |
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| E158 tub forward primer (for integration diagnostics of all β-tub integration vectors) |
PM-112 | 2,5 nmol | 22,00 |
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| E159 tub reverse primer (for integration diagnostics of all β-tub integration vectors) |
PM-113 | 2,5 nmol | 22,00 |
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| A1304 odc forward primer (for integration diagnostics of all odc integration vectors) |
PM-114 | 2,5 nmol | 22,00 |
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| P1510 odc reverse primer (for integration diagnostics of all odc integration vectors) |
PM-115 | 2,5 nmol | 22,00 |
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| H4132 Insert sequencing forward primer (for pLEXSY_invitro-1 expression vector) |
PM-116 | 2,5 nmol | 22,00 |
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| H4134 Forward primer for overlap extension PCR (for LEXSY cell-free protein expression kit) |
PM-117 | 0,5 nmol | 22,00 |
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