Catalog Standard PCR - Thermophilic PolymerasesStandard PCR - Thermophilic Polymerases
 
 
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Standard PCR Thermophilic Polymerases
Thermophilic Polymerases for Maximum Flexibility

Jena Bioscience offers a broad range of optimized thermophilic polymerases. Make your selection from Taq polymerases for routine PCR applications, hot start polymerases for high specific amplifications or proofreading enzyme blends for high fidelity and long range PCR. All enzymes ensure reliable, high performance results and guarantee maximum success for their particular application.

Standard PCR - Product Selection Guide
 

Taq Pol
Thermostable DNA polymerase
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-202S 200 units 35,00    Add this product to your notepad  
L pack PCR-202L 1000 units 140,00    Add this product to your notepad
red - S pack PCR-202S-red 200 units 35,00    Add this product to your notepad
red - L pack PCR-202L-red 1000 units 140,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Taq Pol (5 units/l)
Taq Reaction Buffer complete - 10 x conc.
MgCl2 Stock Solution
 
Taq Pol / high yield
Thermostable DNA polymerase
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-201S 200 units 35,00    Add this product to your notepad  
L pack PCR-201L 1000 units 140,00    Add this product to your notepad
red - S pack PCR-201S-red 200 units 35,00    Add this product to your notepad
red - L pack PCR-201L-red 1000 units 140,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Taq Pol (5 units/l)
High Yield Buffer complete - 10 x conc.
MgCl2 Stock Solution
Taq Pol / high yield is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
The enzyme replicates DNA at 72C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exo-nuclease replacement activity but lacks a 3'→5' exonuclease activity.
Taq Pol - red / high yield buffer contains an inert red dye for easier handling and to facilitate the preparation of the master mix.
 
Hot Start Pol
Heat-activatable DNA polymerase for high specificity
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-203S 200 units 70,00    Add this product to your notepad  
L pack PCR-203L 1000 units 280,00    Add this product to your notepad
red - S pack PCR-203S-red 200 units 70,00    Add this product to your notepad
red - L pack PCR-203L-red 1000 units 280,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Hot Start Pol (5 units/l)
Hot Start Buffer complete - 10 x conc.
MgCl2 Stock Solution
Hot Start Pol provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.
Hot Start Pol requires no prolonged activation step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Hot Start Pol - red contains an inert red dye for easier handling and to facilitate the preparation of the master mix.
 
High Fidelity Pol
Thermostable DNA polymerase for high accuracy
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-204S 100 units 48,00    Add this product to your notepad  
L pack PCR-204L 500 units 192,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Pol (2.5 units/l)
High Fidelity Buffer - 10 x
High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.
 
High Fidelity Hot Start Pol
Heat-activatable DNA polymerase for high accuracy and specificity
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-205S 100 units 68,00    Add this product to your notepad  
L pack PCR-205L 500 units 272,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Hot Start Pol (2.5 units/l)
High Fidelity Buffer - 10 x conc.
High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.
 
Pfu-X Polymerase
Proofreading DNA polymerase for highest accuracy
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-207S 100 units 60,00    Add this product to your notepad  
L pack PCR-207L 500 units 240,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Pfu-X Polymerase (2.5 units/l)
Pfu-X Buffer - 10 x conc.
Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.
 
Sequencing Pol
Taq Pol mutant for incorporation of ddNTPs
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-206S 200 units 70,00    Add this product to your notepad  
L pack PCR-206L 1000 units 280,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Sequencing Pol (5 unis/l)
Sequencing Buffer without MgCl2 - 10 x conc.
MgCl2 Stock Solution
Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium and has a 5'→3' polymerisation-dependent exonuclease replacement activity only.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com