Catalog Cloning and Mutagenesis - Random Mutagenesis KitsCloning and Mutagenesis - Random Mutagenesis Kits
 
 
Cloning Kit   |   Random Mutagenesis Kits
 
Mutagenesis Kits
Molecular Evolution: Random Mutagenesis

  • Three ready-to-go kits for random mutagenesis of a gene of interest
  • Accompanied by detailed manuals and background information
Within three billion years of evolution, nature has produced a plethora of proteins simply by repeated cycles of random mutagenesis followed by in vivo selection. This example of natural evolution has guided researchers to develop strategies for in vitro permutation of genes and proteins. Among a variety of strategies, three powerful techniques have emerged:

Mutagenesis by dNTP Analogs
The method achieves mutagenicity of up to 20% and is based on incorporation of mutagenic dNTP analogs into an amplified DNA fragment. The analogs are eliminated by a second PCR in the presence of the four natural dNTPs, leaving highly mutated DNA ready for further investigation.

Mutagenesis by Error-Prone PCR
Mutagenesis is performed by a PCR reaction under modified conditions that induce an increased error-rate of the DNA-polymerase. Simply run the PCR protocol provided in the manual and achieve mutagenicity in the range of 0.6-2.0% in a single PCR reaction!

Mutagenesis by DNA Shuffling
DNA shuffling generates libraries by random fragmentation of one gene or a pool of related genes, followed by reassembly of the fragments in a self-priming PCR reaction. The mutagenicity is similar to error-prone PCR but DNA shuffling allows recombination of sequences from different, related genes.
 

JBS dNTP-Mutagenesis Kit
Random Mutagenesis by dNTP Analogs
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PP-101 15 reactions 240,00    Add this product to your notepad  

Store at -20C, avoid frequent thawing and freezing

Polymerase
10x Mutagenesis buffer
dNTP mix
dPTP
8-oxo-dGTP
PCR grade water
JBS dNTP-Mutagenesis Kit is based on the incorporation of the mutagenic dNTP analogs 8-oxo-dGTP and dPTP into an amplified DNA fragment by PCR. The mutagenic dNTPs are eliminated by a second PCR step in the presence of the four natural dNTPs only, resulting in a rate of mutagenesis of up to 20%.
 
JBS Error-Prone Kit
Random Mutagenesis by Error-Prone PCR
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PP-102 15 reactions 190,00    Add this product to your notepad  

Store at -20C, avoid frequent thawing and freezing

Polymerase
10x Reaction buffer
10x Error-prone solution
dNTP error-prone mix
PCR grade water
JBS Error-Prone Kit introduces mutations in the gene of interest using modified PCR reaction conditions. An increased MgCl2 concentration, the addition of MnCl2 and the unbalanced ratio of dNTPs induce an increased error-rate of the DNA-polymerase. The rate of mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0%.
 
JBS DNA-Shuffling Kit
Random Mutagenesis by DNA Shuffling
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PP-103 15 reactions 240,00    Add this product to your notepad  

Store at -20C, avoid frequent thawing and freezing

DNase I
10x Digestion buffer
DNase stop solution
Polymerase
10x Shuffling buffer
dNTP mix
PCR grade water
JBS DNA-Shuffling Kit is designed to generate gene libraries by random fragmentation of one gene or a pool of related genes. A controlled fragmentation of the DNA is followed by a random reassembly in a self-priming PCR reaction. This method allows the recombination of sequences from different, related genes. The overall rate of mutagenesis is approx. 0.7%.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com