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Unprotected 2'-Deoxy-nucleosides - Alkyne-containing Deoxy-NucleosidesNon-modified Deoxy-nucleosides
| 2'-Amino-2'-deoxy-Nucleosides
| 5-Propynyl-deoxy-nucleosides
| 7-Deaza-deoxy-nucleosides
| Alpha-deoxy-nucleosides
| Alkyne-containing Deoxy-Nucleosides
| Beta-L-deoxy-nucleosides
| Etheno-deoxy-nucleosides
| Halogenated Deoxy-Nucleosides
| Methylated Deoxy-nucleosides
| Thio-deoxy-nucleosides
| Other Modified Deoxy-nucleosides
Nucleosides containing reactive groups (triple bonds) allow the attachment of reporter groups such as biotin or fluorescent dyes by means of click chemistry.
As EdU (5-Ethynyl-2'-deoxyuridine), a nucleoside analog of thymidine, is incorporated by proliferating cells, subsequent incubation of the cells with fluorescent azide facilitates the direct measurement of de novo DNA synthesis [1],[2],[3]. It can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine). Analog 5-Ethynyl-2'-deoxy-cytidine can be used to detect DNA synthesis with similar sensitivity as EdU [5].
Based on the same principle, 5-EU (5-Ethynyl-uridine) can be used for detection of RNA transcription and turnover in vivo. As it is incorporated into RNA transcripts, 5-EU-labeled cellular RNA can be detected quickly and with high sensitivity with fluorescent azides [4].
As EdU (5-Ethynyl-2'-deoxyuridine), a nucleoside analog of thymidine, is incorporated by proliferating cells, subsequent incubation of the cells with fluorescent azide facilitates the direct measurement of de novo DNA synthesis [1],[2],[3]. It can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine). Analog 5-Ethynyl-2'-deoxy-cytidine can be used to detect DNA synthesis with similar sensitivity as EdU [5].
Based on the same principle, 5-EU (5-Ethynyl-uridine) can be used for detection of RNA transcription and turnover in vivo. As it is incorporated into RNA transcripts, 5-EU-labeled cellular RNA can be detected quickly and with high sensitivity with fluorescent azides [4].
| 5-Ethynyl-2'-deoxy-cytidine (5-EdC) | |||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| 10 mg pack | CLK-N003-10 | 10 mg | 96,50 | 
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| 5-Ethynyl-2'-deoxy-uridine (5-EdU) | |||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| 5 mg pack | CLK-N001-5 | 5 mg | 30,00 |
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| 25 mg pack | CLK-N001-25 | 25 mg | 96,50 |
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| 500 mg pack | CLK-N001-500 | 500 mg | 885,00 |
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| 5 g pack | CLK-N001-5000 | 5 g | 3.325,00 |
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Selected references:
[1] Salic et al. (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. USA 105 (7):2415.
[2] Doerr (2008) DNA synthesis lights up. Nature Methods 5 (4):286.
[3] Chehrehasa et al. (2009) EdU, a new thymidine analog for labeling proliferating cells in the nervous system. Journal of Neuroscience Methods 177:122.
[4] Jao et al. (2009) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Nat. Acad. Sci. USA 105 (41):15779.
[5] Qu et al. (2011) 5-Ethynyl-2'-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells. Anal Biochem 417(1):112.
[1] Salic et al. (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. USA 105 (7):2415.
[2] Doerr (2008) DNA synthesis lights up. Nature Methods 5 (4):286.
[3] Chehrehasa et al. (2009) EdU, a new thymidine analog for labeling proliferating cells in the nervous system. Journal of Neuroscience Methods 177:122.
[4] Jao et al. (2009) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Nat. Acad. Sci. USA 105 (41):15779.
[5] Qu et al. (2011) 5-Ethynyl-2'-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells. Anal Biochem 417(1):112.
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