Fluorescent Labeling by PCR  |  Fluorescent Labeling by Nick Translation  |  Biotin/Digoxigenin Labeling by PCR

Fluorescent Labeling by PCR

More Fluorescent Nucleotides for a wide range of applications can be found here!

Emission Color	Product (Labeling Kit / Dye-Nucleotide	Absorption max. [nm]	Emission max. [nm]	Labeling Rate *	Replacement for
blue	Atto425 PCR Labeling Master Atto425 PCR Labeling Kit Atto425-dUTP-PCR	436	484	5%	DEAC: 430 / 477 nm
green	Atto488 PCR Labeling Master Atto488 PCR Labeling Kit Atto488-dUTP-PCR	501	523	9%	Alexa Fluor 488: 495 / 519 nm Fluorescein: 495 / 520 nm FAM: 495 / 520 nm Rhodamine 110: 496 / 520 nm Oregon Green 514: 506 / 526 nm Rhodamine green: 503 / 528 nm Rhodamine 123: 507 / 529 nm
yellow- green	Atto532 PCR Labeling Master Atto532 PCR Labeling Kit Atto532-dUTP-PCR	532	553	10%	Alexa Fluor 514: 517 / 542 nm JOE: 520 / 548 nm TET: 521 / 542 nm Carboxyrhodamine 6G: 525/547nm Alexa Fluor 532: 534 / 553 nm HEX: 535 / 556 nm
yellow	Cy3 PCR Labeling Master Cy3 PCR Labeling Kit Cy3-dUTP-PCR Atto550 PCR Labeling Master Atto550 PCR Labeling Kit Atto550-dUTP-PCR	550 554	570 576	6% 10%	Alexa Fluor 555: 555 / 565 nm Alexa Fluor 546: 556 / 573 nm TAMRA: 546 / 576 nm Rhodamine Red: 560 / 580 nm Spectrum Orange: 559 / 588 nm
orange	TexasRed PCR Labeling Master TexasRed PCR Labeling Kit TexasRed-dUTP-PCR	583	603	6%	Rhodamine ITC: 572 / 596 nm Cy 3.5: 581 / 596 nm ROX: 576 / 601 nm Alexa Fluor 568: 578 / 603 nm
orange	Atto590 PCR Labeling Master Atto590 PCR Labeling Kit Atto590-dUTP-PCR	594	624	8%	Alexa Fluor 594: 590 / 617 nm Alexa Fluor 610: 612 / 628 nm
red	Cy5 PCR Labeling Master Cy5 PCR Labeling Kit Cy5-dUTP-PCR	643	667	6%	Alexa Fluor 647: 650 / 665 nm
far-red	Atto655 PCR Labeling Master Atto655 PCR Labeling Kit Atto655-dUTP-PCR	663	684	3%	Alexa Fluor 660: 664 / 691 nm Cy 5.5: 675 / 694 nm

* Labeling Rate: A labeling rate of 10% corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 100 dTTPs or, respectively, into a 200 bp PCR fragment if an equal distribution of dATP, dCTP, dGTP and dTTP in the fragment can be assumed.

Visible spectrum of light