Fluorescent Labeling by PCR  |  Fluorescent Labeling by Nick Translation  |  Biotin/Digoxigenin Labeling by PCR

 

Fluorescent Labeling by PCR



 
The corresponding and further fluorescent dUTPs can be found here!

Emission ColorProduct
(Labeling Kit / Dye-Nucleotide
Absorption max. [nm]Emission max. [nm]Labeling Rate *Replacement for
blue
Atto425 PCR Labeling Kit

4364845%DEAC: 430 / 477 nm
greenAtto488 PCR Labeling Kit
5015239%Alexa Fluor 488: 495 / 519 nm
Fluorescein: 495 / 520 nm
FAM: 495 / 520 nm
Rhodamine 110: 496 / 520 nm
Oregon Green 514: 506 / 526 nm
Rhodamine green: 503 / 528 nm
Rhodamine 123: 507 / 529 nm
yellow- greenAtto532 PCR Labeling Kit
53255310%Alexa Fluor 514: 517 / 542 nm
JOE: 520 / 548 nm
TET: 521 / 542 nm
Carboxyrhodamine 6G: 525/547nm
Alexa Fluor 532: 534 / 553 nm
HEX: 535 / 556 nm
yellowCy3 PCR Labeling Kit
5505706%Alexa Fluor 555: 555 / 565 nm
Alexa Fluor 546: 556 / 573 nm
TAMRA: 546 / 576 nm
Rhodamine Red: 560 / 580 nm
Spectrum Orange: 559 / 588 nm
orangeTexasRed PCR Labeling Kit
5836036%Rhodamine ITC: 572 / 596 nm
Cy 3.5: 581 / 596 nm
ROX: 576 / 601 nm
Alexa Fluor 568: 578 / 603 nm
orange
Atto590 PCR Labeling Kit

5946248%Alexa Fluor 594: 590 / 617 nm
Alexa Fluor 610: 612 / 628 nm
red
Cy5 PCR Labeling Kit

6436676%Alexa Fluor 647: 650 / 665 nm
far-red
Atto655 PCR Labeling Kit

6636843%Alexa Fluor 660: 664 / 691 nm
Cy 5.5: 675 / 694 nm

 
* Labeling Rate: A labeling rate of 10% corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 100 dTTPs or, respectively, into a 200 bp PCR fragment if an equal distribution of dATP, dCTP, dGTP and dTTP in the fragment can be assumed.


 

Visible spectrum of light

spectrum of light