The ability to purify recombinant proteins using affinity chromatography has greatly advanced protein research.

In affinity chromatography, the protein of interest is purified by its ability to bind a specific ligand that is immobilized on a chromatographic bead material (matrix).

Crude cell-lysates are incubated with such a matrix under conditions that ensure specific binding of the protein to the immobilized ligand. Other proteins that do not bind the immobilized ligand are washed through. Elution of the bound protein of interest can be achieved by changing the experimental conditions to favour desorption.

Jena Bioscience offers both Agarose and Magnetic Beads as matrix for different purification tasks.

Also available: Auxilary Equipment such as empty Columns, Spin Columns or Cartridges.

Click on the links below to visit the appropriate product sections:

 
Purification ofAgaroseMagnetic Beads
Nucleotide-binding proteinsNTP/NDP/NMP Agaroses
mRNA cap binding proteinsm7GTP Agarose
KinasesATP Agaroses
Phospho Amino Acid binding proteinsO-Phospho-Serine Agarose
O-Phospho-Threonine Agarose
O-Phospho-Tyrosine Agarose

GST-tagged proteinsGlutathione AgaroseGlutathione Magnetic Beads
His-tagged proteinsNi-NTA Agarose (fast flow)
Ni-IDA Agarose (fast flow)
Co-IDA Agarose (fast flow)
Ni-, Zn-, Co-, Cu-IDA Agarose (gravity flow)
Ni-NTA Magnetic Beads
Co-NTA Magnetic Beads
Ni-IDA Magnetic Beads
Co-IDA Magnetic Beads
AntibodiesProtein A Agarose
Protein G Agarose

Alkyne- or DBCO-functionalized proteinsAzide AgaroseAzide Magnetic Beads
Azide-functionalized proteinsAlkyne Agarose
DBCO Agarose
Alkyne Magnetic Beads
DBCO Magnetic Beads

 
 
 
Affinity Chromatography Mini Catalog

  PDF download Download the Affinity Chromatography Mini Catalog in PDF format (2.9 MB)