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Catalog Applications for Modified Nucleotides - DNA ManipulationApplications for Modified Nucleotides - DNA Manipulation
 
 
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DNA Analysis   |   DNA Interaction Studies   |   DNA Manipulation
 
Random Mutagenesis   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
8-Oxo-dGTP, S pack NU-1117S 30 Units 112,74    Add this product to your notepad  
8-Oxo-dGTP, L pack NU-1117L 150 Units 330,23    Add this product to your notepad  
dPTP, S pack NU-1119S 30 Units 112,74    Add this product to your notepad  
dPTP, L pack NU-1119L 150 Units 330,23    Add this product to your notepad  
dITP - solution, pack
100 mM		 NU-1007 1 ml
(100 µmol)		 72,00    Add this product to your notepad  
5Br-dUTP (5-BrdUTP), S pack NU-122S 50 Units 112,74    Add this product to your notepad  
5Br-dUTP (5-BrdUTP), L pack NU-122L 250 Units 330,23    Add this product to your notepad  

Description
Modified nucleotides for random mutagenesis including
lethal or HIV mutagenesis
Incorporation
Taq DNA Polymerase
Reverse Transcriptase (8-Oxo-dGTP)

References
Zaccolo et al. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. Journal of Molecular Biology 285(2):775
Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589
dITP:
Dierick et al. (1993) Incorporation of dITP or 7-deaza dGTP during PCR improves sequencing of the product. Nucleic Acids Research 21-18:4427
5Br-dUTP:
Ma et al. (2008) The mutagenic properties of BrdUTP in a random mutagenesis process. Mol. Biol. Rep. 35:663
Lethal mutagenesis:
Loeb et al. (1999) Lethal mutagenesis of HIV with mutagenic nucleoside analogs. Proc. Natl. Acad. Sci. USA 96:1492
 

Inactivation of Restriction Enzyme Recognition Sites   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
5-Methyl-dCTP, S pack NU-1125S 100 Units 112,74    Add this product to your notepad  
5-Methyl-dCTP, L pack NU-1125L 500 Units 330,23    Add this product to your notepad  
5-AcOHg-dCTP, S pack NU-1149S 100 Units 107,37    Add this product to your notepad  
5-AcOHg-dCTP, L pack NU-1149L 500 Units 314,50    Add this product to your notepad  

Description
5-Methyl-dCTP is widely used for construction of cDNA libraries
Incorporation of 5-Methyl-dCTP
M-MuLV Reverse Transcriptase
Klenow Fragment of DNA Polymerase I
Sequenase DNA Polymerase
(Taq Polymerase, Vent) *
Incorporation of Hg-dCTP
DNA Polymerase I

References to 5-Methyl-dCTP
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584.
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 (3):681.
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research 6:369.
* Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 (5):1081.
Reference to Hg-dCTP
Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445.
 

Inhibition of DNA Methylation   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
5-Aza-dCTP, S pack
(Decitabine triphosphate)		 NU-1118S 20 Units 112,74    Add this product to your notepad  
5-Aza-dCTP, L pack
(Decitabine triphosphate)		 NU-1118L 100 Units 330,23    Add this product to your notepad  

Description
5-Aza-dCTP is an inhibitor of DNA methylation and antileukemic agent. It has been used to reactivate silent tumor suppressor
genes.
Incorporation
DNA Polymerase alpha

References
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Molecular Pharmacology 24 (1):109.
Lemaire et al. (2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. Anti-Cancer Drugs 16 (3):301.
 

Selective RNA Amplification   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
dITP - solution, pack
100 mM		 NU-1007 1 ml
(100 µmol)		 72,00    Add this product to your notepad  

Description
The nucleotide allows a selective RNA amplification in the
presence of genomic DNA by shifting the strand separation temperature
Incorporation
Tth Polymerase

Reference
Auer et al. (1996) Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase. Nucl. Acids Res. 24 (24):5021.
 

Prevention of Transcription Arrests   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
5Br-dUTP (5-BrdUTP), S pack NU-122S 50 Units 112,74    Add this product to your notepad  
5Br-dUTP (5-BrdUTP), L pack NU-122L 250 Units 330,23    Add this product to your notepad  

Description
Modified nucleotides for higher efficiency of readthrough and prevention of transcription arrests
Incorporation
RNA Polymerase II
E. coli RNA Polymerase

References
Mote et al. (1998) Recognition of a Human Arrest Site Is Conserved between RNA Polymerase II and Prokaryotic RNA Polymerases. J. Biological Chemistry 273 (27):16843.
Pal et al. (2002) Strong Natural Pausing by RNA Polymerase II within 10 Bases of Transcription Start May Result in Repeated Slippage and Reextension of the Nascent RNA. Molecular and Cellular Biology 22 (1):30.
 

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