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Protein Labeling - Site-specific labeling of C- and/or N-terminus Site-specific labeling of C- and/or N-terminus
| Amine and Thiol Protein labeling Kits
| Luminescent Protein Labeling
The novel labeling kits presented here are designed for site-specific labeling of
- any protein’s C-terminus using convenient, fast, mild and biocompatible oxyamine–ketone ligation (scheme),
- an N-terminal cysteine using native chemical ligation with a fluorophore-thioester, or
- a protein’s C & N-termini simultaneously with two colors in a One-Pot Dual Color labeling reaction for fluorescence resonance energy transfer (FRET) studies
General method for protein modification at the C terminus: Under mild and biocompatible conditions a protein thioester is transformed with 1,2-bis(oxyamino)ethane into an oxyamino-modified species that in a second step reacts rapidly with ketones carrying a fluorescent label.
(adapted from Yi et al. (2010))
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| C-terminal Protein Labeling Kit Universal C-terminal Protein Labeling with Coumarin |
FP-301-COU | 1 Kit (for labeling of 5 mg protein) |
320,00 | 
 |
 |
| N-terminal Protein Labeling Kit Universal N-terminal Protein Labeling with Coumarin |
FP-302-COU | 1 Kit (for labeling of 5 mg protein) |
380,00 |
|
|
| Protein Dual-Labeling Kit Universal C/N-terminal Protein Labeling with Fluorescein/Coumarin |
FP-303-FLU | 1 Kit (for labeling of 5 mg protein) |
520,00 |
|
|
Selected References
Yi, L. et al. (2010) A Highly Efficient Strategy for Modification of Proteins at the C Terminus. Angewandte Chemie Int. Ed. 49:1-6
Yi, L. et al. (2011) One-Pot Dual-Labeling of a Protein via Two Chemoselective Reactions. Angewandte Chemie Int. Ed. 50:8287-90
Yi, L. et al. (2011) One-Pot Dual-Labeling of a Protein via Two Chemoselective Reactions. Angewandte Chemie Int. Ed. 50:8287-90