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Catalog His-tagged Proteins - Nickel NTA Agaroses, Fast FlowHis-tagged Proteins - Nickel NTA Agaroses, Fast Flow
 
 
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Suitable for FPLC, gravity flow and batch purification

His-tagged proteins are efficiently purified by a one-step procedure from crude lysates both under denaturing and non-denaturing conditions
 


  • High capacity
  • Minimum metal leaching

Product characteristics

Matrix6 % cross-linked agarose
Bead size50-170 µm
LinkerNitrilotriacetic acid (NTA)
Binding capacity*> 50 mg/ml gel
Recommended flow rate1 ml column – 1.0 ml/min
10 ml column – 5.0 ml/min
Maximum linear flow rate300 cm/h
Recommended pHbetween 7 - 8
Chemical stabilityStable to all solutions commonly used in gel filtration including reducing agents (see procedure)

* Tested with 6xHis–GFPuv.
Binding capacity will differ for each target protein.
 

Ni-NTA Agarose    
Product Cat. No. Amount Price (EUR) Buy / Note  
bulk material - 10 ml AC-501-10 10 ml 97,00    Add this product to your notepad  
bulk material - 25 ml AC-501-25 25 ml 231,00    Add this product to your notepad
bulk material - 100 ml AC-501-100 100 ml 867,00    Add this product to your notepad
bulk material - 500 ml AC-501-500 500 ml 3.400,00    Add this product to your notepad

 
Please contact us at info@jenabioscience.com for more information.