Jena Bioscience distributes evocatal's anaerobic fluorescent proteins
Fluorescent reporter proteins are valuable noninvasive molecular tools for in vivo real-time imaging of living cells and tissues as well as in vitro fluorescence labeling. A major drawback of existing GFP-like reporter proteins is their strict requirement for molecular oxygen as a cofactor for the synthesis of their respective chromophores. Therefore, the application in anaerobic systems is not possible.
The flavin mononucleotide (FMN)-based fluorescent proteins (FbFPs) from the evoglow^® series were developed to overcome these restrictions. The evoglow^® proteins can be used as fluorescent reporters in both aerobic and anaerobic biological systems.


The designation evoglow^® comprises a novel type of fluorescent proteins containing a Flavin-mono-nucleotide-based cofactor. They are thus capable of developing bright cyan-green fluorescence even in the complete absence of oxygen. They fold rapidly in vivo and thus enable immediate detection of expression to study anaerobic inter- and intracellular processes. Since their fluorescence intensity as well as the excitation and emission spectra of the evoglow^® proteins lie within the same range as those of other fluorescent proteins, the same instrumental setup can be used for their detection and analysis as for other fluorescent proteins.

evoglow^® Plasmid Kits

The evoglow^® Plasmid Kits allow easy cloning and expression of the evoglow^® genes.

evoglow^® Antibodies

evoglow^® antibodies are approved for the specific detection of the fluorescence reporters by Western Blot.