HIT OPTIMIZATION

Strategies to optimize initial crystallization hits:

 
Thermodynamics/KineticsInput ProteinChemical EnvironmentOptimization - Flow Chart
 


Optimization - Chemical Environment

Chemical Environment

Kits and screens to increase protein solubility and stability prior to crystallization as well as screens to optimize the chemical composition of your crystallization reagents, i.e. buffer pH, precipitant and additive composition.

 


Optimization - Input Protein

Input Protein

Get your protein ready for crystallization! Consider to alter the protein surface or modify the sequence by truncation or mutagenesis. Or try a new mode of production.

 


Optimization - Thermodynamics / Kinetics

Thermodynamics / Kinetics

Do not discard bad diffracting crystals! They can be very useful, too. Try to improve diffraction by varying the solvent content or use your initial crystals for seeding.