Catalog JBScreen Family - JBScreen PentaerythritolJBScreen Family - JBScreen Pentaerythritol
 
 
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JBScreen Classic   |   JBScreen Basic   |   JBScreen Membrane   |   JBScreen Kinase   |   JBScreen Nuc-Pro   |   JBScreen PEG/Salt   |   JBScreen Pentaerythritol   |   JBScreen PACT++   |   JBScreen JCSG++   |   Pi-minimal Screen   |   Pi-PEG Screen   |   JBScreen Wizard
 
JBScreen bulk & HTS

JBScreen Pentaerythritol has been designed for efficient crystallization screening of biological macromolecules based on pentaerythritol polymers as precipitants. The screen was developed by Ulrike Demmer from the Max-Planck-Institute for Biophysics in Frankfurt.

The choice of a suitable precipitant is of crucial importance for the crystallization of proteins. JBScreen Pentaerythritol utilizes two novel precipitating agents, i.e. pentaerythritol propoxylate and pentaerythritol ethoxylate. Both are branched polymers containing a pentaerythritol backbone. Thus they differ from more traditional precipitants like MPD and PEG’s in size and nature.

In addition, pentaerythritol polymers function as cryoprotectants. Protein crystals grown in high concentrations of these precipitants can be frozen directly from the crystallization drop. The successful application of pentaerythritol polymers to yield protein crystals was first described by Gulick et al. [1]. Now this class of precipitants has been used for membrane crystallization, too. The X-ray structure of cbb3 Cytochrome Oxidase was recently published in Science [2]. Crystals of this proton pumping membrane protein were successfully grown using pentaerythritol ethoxylate as precipitation agent.

 
JBScreen Pentaerythritol comprises of 96 unique conditions, based on 4 different pentaerythritol polymers as precipitating agent:
  • Pentaerythritol propoxylate 426 (5/4 PO/OH)
  • Pentaerythritol propoxylate 629 (17/8 PO/OH)
  • Pentaerythritol ethoxylate 270 (3/4 EO/OH)
  • Pentaerythritol ethoxylate 797 (15/4 EO/OH)
The 4 polymers are arranged to a grid screen, thus allowing screening i) of three different precipitant concentrations, ii) four different pH values and iii) with and without the addition of salts, i.e. magnesium chloride, ammonium sulfate, potassium chloride.
The advantage of JBScreen Pentaerythritol not only lies in the novel 96 conditions but also in the systematic arrangement of the unique reagents, which enables the user to compare individual conditions directly. Even if initial screening may not always yield crystals, valuable information about the protein under investigation can be obtained from the scoring sheet.
 


Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
 
 
 
 
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
JBScreen Pentaerythritol 1
(PEP 426 based)
CS-191 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Pentaerythritol 2
(PEP 629 based)
CS-192 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Pentaerythritol 3
(PEE 270 based)
CS-193 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Pentaerythritol 4
(PEE 797 based)
CS-194 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Pentaerythritol 1 - 4 CS-195 4 Kits 450,00    Add this product to your notepad
JBScreen Pentaerythritol HTS CS-210L 96 solutions
(1,7 ml each)
225,00    Add this product to your notepad      

References

[1] Gulick et al. (2002) Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase. Acta Cryst. D58:306.
[2] Buschmann et al. (2010) The Structure of cbb3 Cytochrome Oxidase Provides Insights into Proton Pumping. Science 329:327.

 

Selected Literature Citations of JBScreen Pentaerythritol

  • Rekittke et al. (2012) Structure of the GcpE (IspG)–MEcPP complex from Thermus thermophilus. FEBS Letters 586(19):3452.
  • Fislage et al. (2011) Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus. Acta Cryst. F 67(2):1432.
 


Please contact xtals@jenabioscience.com with questions or inquiries.