Catalog Real-Time PCR - qPCR SupplementsReal-Time PCR - qPCR Supplements
 
 
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qPCR Mixes for Dual Labeled Fluorescent Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 
qPCR Supplements
qPCR Supplements

Helpful tools for Real-Time PCR, to test the function or control the quality of qPCR polymerases and qPCR assay conditons.
 

Fluorescein Reference Dye
Reference dye for fluorescence signal normalization
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-355 1000 reactions
(500 l)
25,00    Add this product to your notepad  

Store at -20C in the dark

100x Fluorescein Reference Dye, 500 l
Fluorescein Reference Dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the FAM reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 100x concentration.
 
ROX Reference Dye
Reference dye for fluorescence signal normalization
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-351 500 reactions
(500 l)
25,00    Add this product to your notepad  

Store at -20C in the dark

50x ROX reference dye, 500 l
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
 
EvaGreen™ Fluorescent DNA Stain
DNA Intercalating dye for real-time qPCR fluorescence detection
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-352 500 reactions
(500 l)
40,00    Add this product to your notepad  

Store at -20C in the dark

50x EvaGreenTM Fluorescent DNA Stain, 500 l
EvaGreenTM Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melt curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
The high quantum yield, excellent stability and less inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications as replacement for the widely used SYBR Green I dye.
 
Thermolabile UNG (Uracil N-Glycosylase)
Prevention of carry-over contaminations
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-353 200 units 100,00    Add this product to your notepad  

Store at -20C

Uracil N-Glycosylase, 200 units (1 unit/l)
Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures.
UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions.
 
qPCR Control Kit    
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-354 500 reactions 130,00    Add this product to your notepad  

Store at -20C
beta-actin Primer-Probe Mix must be stored in the dark

beta-actin Primer-Probe Mix
Human genomic DNA (male)
PCR Control Kit is designed as tool to confirm or compare the quality and functionality of quantitative real-time analysis using DNA probe based detection. The kit contains a forward / reverse primer-pair and the dual labeled fluorescent probe designed to detect the β-actin gene from human genomic DNA.
 

EvaGreen is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.