Catalog RNA Labeling - Fluorescent RNA Labeling RNA Labeling - Fluorescent RNA Labeling
 
 
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Fluorescent RNA Labeling   |   Azide RNA Labeling
 

RNA Labeling Kits

Jena Bioscience RNA Labeling Kits allow fluorescent labeling of RNA probes in less than two hours.
Thoroughly selected fluorophores are linked to UTP resulting in fluorescent nucleotides with superior properties such as high stability, high signal to noise ratios and high quantum yields. Our RNA Labeling Kits ensure efficient incorporation using T7 in vitro transcription. They are optimized for transcription efficiency and a high degree of labeling.
Typical RNA yields and degrees of labeling (DOL) are given in the table below. Experiments were performed using 500 ng template DNA (523 bp λ-DNA fragment containing a T7 promoter site) in a 20 l reaction assay.
 
Emission ColorFluorophoreEx/Em [nm]YieldDOL
greenAtto 488501/5232 g16.5
yellow-greenAtto 532532/5533 g3.3
yellowAtto 550554/5761.5 g2.9
redAtto 647N644/6692 g1.5
far-redAtto 680680/7002.5 g2.2
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
Atto 488 RNA Labeling Kit
Fluorescent labeling of RNA probes
FP-220-488 10 reactions 428,00    Add this product to your notepad  
Atto 532 RNA Labeling Kit
Fluorescent labeling of RNA probes
FP-220-532 10 reactions 428,00    Add this product to your notepad  
Atto 550 RNA Labeling Kit
Fluorescent labeling of RNA probes
FP-220-550 10 reactions 428,00    Add this product to your notepad  
Atto 647N RNA Labeling Kit
Fluorescent labeling of RNA probes
FP-220-647N 10 reactions 428,00    Add this product to your notepad  
Atto 680 RNA Labeling Kit
Fluorescent labeling of RNA probes
FP-220-680 10 reactions 428,00    Add this product to your notepad  

Selected References

[1] Uri et al. (2012) Bisubstrate fluorescent probes and biosensors in binding assays for HTS of protein kinase inhibitors. Biochimica et Biophysica Acta 1804:541.
[2] Vaasa et al. (2009) High-affinity bisubstrate probe for fluorescence anisotropy binding/displacement assays with protein kinases PKA and ROCK. Analytical Biochemistry 385(1):85.
[3] Enkvist et al. (2012) Protein-Induced Long Lifetime Luminescence of Nonmetal Probes. ACS chemical biology 6:1052.
 
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