Catalog Standard PCR - SupplementsStandard PCR - Supplements
 
 
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Gel Loading Buffer
Standard PCR Supplements

Convenient tools designed to save you valuable time at the bench and yielding superior results and performance. The Kits are recommended to facilitate the amplification of GC-rich structures, to enhance the yield and to serve as an internal lab standard.

Standard PCR - Product Selection Guide
 

Gel Loading Buffer with DNA Stain
Loading buffer for agarose or polyacrylamide gels with EvaGreenTM fluorescent DNA stain
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Blue PCR-255-bl 5x 1 ml 35,00    Add this product to your notepad  
Green PCR-255-gr 5x 1 ml 35,00    Add this product to your notepad
Orange PCR-255-or 5x 1 ml 35,00    Add this product to your notepad

Store at 2-8C in the dark

6x concentration
Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreenTM DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Its high quantum yield and excellent stability makes it the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR Green.
The buffers contain tracking dyes as indicators for DNA fragment migration.
The Gel Loading Buffers provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR Green.
 
Gel Loading Buffer
Loading buffer for agarose or polyacrylamide gels
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Blue PCR-254-bl 5x 1 ml 25,00    Add this product to your notepad  
Green PCR-254-gr 5x 1 ml 25,00    Add this product to your notepad
Orange PCR-254-or 5x 1 ml 25,00    Add this product to your notepad

Store at 2-8C

6x concentration
Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels.
Blue Gel Loading Buffer contains bromophenol blue and xylene cyanol FF as tracking dyes and indicator for DNA fragment migration. It is recommended for loading of fragment larger than 500 bp.
Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and is recommended for loading of fragment from 100 to 2000 bp.
Orange Gel Loading Buffer contains orange G and is recommended for loading of fragment smaller than 500 bp.
 
EvaGreenTM Fluorescent Gel Stain
Staining dye for DNA gel electrophoresis
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-256 500 l 40,00    Add this product to your notepad  

Store at -20C in the dark

100x concentration
EvaGreenTM Fluorescent Gel Stain is a superior DNA staining dye for DNA analysis in gel electrophoresis applications. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent allowing the detection of lowest DNA amounts. It is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
 
PCR Control Kit
Amplification of a beta-actin gene fragment from human genomic DNA
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-253 500 reactions 50,00    Add this product to your notepad  

Store at -20C

beta-actin Primer Mix
Human genomic DNA (male)
PCR Control Kit is designed for quality and benchmark tests, as positive control or internal lab standard for Taq DNA polymerases. The kit contains a forward / reverse primer-pair designed to amplify a 295 bp long fragment of the β-actin gene from human genomic DNA.
 
Lambda DNA
Positive control templates for PCR
   
Product Cat. No. Amount Price (EUR) Buy / Note  
pack PCR-259 100 g 35,00    Add this product to your notepad  

 
Human Genomic DNA - male
Positive control templates for PCR
   
Product Cat. No. Amount Price (EUR) Buy / Note  
pack PCR-261 50 g 115,00    Add this product to your notepad  

 
Taq Reaction Buffer    
Product Cat. No. Amount Price (EUR) Buy / Note  
complete - 10 x PCR-262-c 5x 1.2 ml 10,00    Add this product to your notepad  
without MgCl2 - 10 x PCR-262-wo 5x 1.2 ml 10,00    Add this product to your notepad

 
High Yield Buffer    
Product Cat. No. Amount Price (EUR) Buy / Note  
complete - 10 x PCR-263-c 5x 1.2 ml 10,00    Add this product to your notepad  
without MgCl2 - 10 x PCR-263-wo 5x 1.2 ml 10,00    Add this product to your notepad

 
Hot Start Buffer    
Product Cat. No. Amount Price (EUR) Buy / Note  
complete - 10 x PCR-264-c 5x 1.2 ml 10,00    Add this product to your notepad  
without MgCl2 - 10 x PCR-264-wo 5x 1.2 ml 10,00    Add this product to your notepad

 
MgCl2 Stock Solution    
Product Cat. No. Amount Price (EUR) Buy / Note  
25 mM PCR-266-25 4x 1.5 ml 10,00    Add this product to your notepad  

 
PCR Additives Kit
Stabilizer and enhancer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
Kit PCR-252 500 reactions 40,00    Add this product to your notepad  

Store at 2-8C or -20C

Taq-stabilizer
GC-enhancer
PCR Additives Kit contains a stabilizer and enhancer bundle for the amplification of difficult templates such as GC-rich or extremly long fragments.
5-10% Taq-stabilizer in the PCR reaction is recommended to improve amplification efficiency and specificity. It stabilizes Taq polymerase and reduces secondary DNA structures.
10-20% GC-enhancer in the PCR is recommended for amplifying GC-rich templates. It reduces the melting temperature of DNA.
 
PCR-grade Water    
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-258S 10x 1,2 ml 14,00    Add this product to your notepad  
L pack PCR-258L 50 ml 16,00    Add this product to your notepad
XL pack PCR-258XL 500 ml 28,00    Add this product to your notepad

Store at 2-8C or -20C

PCR-grade Water
PCR-grade Water is ultrapure, sterile filtered and autoclaved water for use in molecular biology applications like standard PCR, qPCR or RT-PCR. It is treated with DEPC to avoid any residual enzymatic activity.
 
LE Agarose
general use Agarose
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-269S 100 g 90,00    Add this product to your notepad  
L pack PCR-269L 500 g 360,00    Add this product to your notepad

 

For inquiries or further information, please contact us at pcr@jenabioscience.com