JBScreen Classic is a crystallization kit designed for efficient and flexible screening of crystallization conditions for proteins, peptides, nucleic acids, macromolecular complexes and water-soluble small molecules.

The JBScreen Classic Kits 1-10 cover 240 of the most prominent buffers for protein crystallization. Their compositions result from data mining of several thousands of crystallized proteins. JBScreen Classic represents the statistically most successful buffers that yielded protein crystals suitable for X-ray diffraction.

The JBScreen Classic buffers are principally ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient.

JBScreen Classic comprises 10 kits of 24 unique reagents in the standard 10 ml bulk format.

JBScreen Classic HTS I+II contains the formulations of the JBScreen system, adopted to fit the 96-well format for high throughput crystallization applications. Each JBScreen Classic HTS deep-well block is pre-filled with 96 sterile conditions at 1.7 ml each.

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.

   Description how the JBScreen buffers are prepared

• Couturier et al. (2013) In the Absence of Thioredoxins, What Are the Reductants for Peroxiredoxins in Thermotoga maritima. Antioxidants & Redox Signaling 18:1613.
• Volkov et al. (2013) Crystal structure analysis of a fatty acid double-bond hydratase from Lactobacillus acidophilus. Acta Cryst D 69:648.
• Raaf et al. (2013) First Structure of Protein Kinase CK2 Catalytic Subunit with an Effective CK2b-competitive Ligand. ACS Chemical Biology DOI: 10.1021/cb3007133.
• Ferraroni et al. (2013) X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP. Journal of Structural Biology 182:44.
• Fuengfuloy et al. (2013) Overproduction of the N-terminal anticodon-binding domain of the non-discriminating aspartyl-tRNA synthetase from Helicobacter pylori for crystallization and NMR measurements. Protein Expression and Purification 89:25.
• Ielasi et al. (2012) The epithelial adhesin 1 (Epa1p) from the humanpathogenic yeast Candida glabrata: structural and functional study of the carbohydrate-binding domain. Acta Cryst D 68:210.
• Upadhyay et al. (2012) Structure and Catalytic Mechanism of N5,N10-Methenyl-tetrahydromethanopterin Cyclohydrolase. Biochemistry 51:8435.
• Ferraroni et al. (2012) Reaction intermediates and redox state changes in a blue laccase from Steccherinum ochraceum observed by crystallographic high/low X-ray dose experiments. Journal of Inorganic Biochemistry 111:203.
• Ferrero et al. (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the RNA-dependent RNA polymerase from Thosea asigna virus. Acta Cryst. F 68:1263.
• Sharma et al. (2012) Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase. Acta Cryst. F 68:931.
• Tada et al. (2012) Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa. Acta Cryst. F 68:535.
• Badireddy et al. (2011) Cyclic AMP Analog Blocks Kinase Activation by Stabilizing Inactive Conformation: Conformational Selection Highlights a New Concept in Allosteric Inhibitor Design. Mol. Cell. Proteomics 10:M110.004390.
• Husain et al. (2011) Structural basis for the methylation of A1408 in 16S rRNA by a panaminoglycoside resistance methyltransferase NpmA from a clinical isolate and analysis of the NpmA interactions with the 30S ribosomal subunit. Nucleic Acids Research 39(5):1903.
• Patino et al. (2011) Structure Analysis of the IL-5 Ligand-Receptor Complex Reveals a Wrench-like Architecture for IL-5Rα. Structure 19(12):1864.
• Li et al. (2011) Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Crystal Growth & Design 11(2):530.
• Marcia et al. (2009) The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration. PNAS 106:9625.
• Vulliez-LeNormand et al. (2008) Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody. PNAS 105:9976.
• Irimia et al. (2004) Methanoarchaeal sulfolactate dehydrogenase: prototype of a new family of NADH-dependent enzymes. EMBO J. 23:1234.
• Vallazza et al. (2004) First look at RNA in L-configuration. Acta Cryst. D 60:1.
• Kundrot (2004) Which strategy for a protein crystallization project? Cell. Mol. Life Sci. 61:525.
• Vorup-Jensen et al. (2003) Structure and allosteric regulation of the aXb2 integrin I domain. PNAS 100:1873.

Please contact xtals@jenabioscience.com with questions or inquiries.