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Catalog Applications for Modified Nucleotides - DNA AnalysisApplications for Modified Nucleotides - DNA Analysis
 
 
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DNA Analysis   |   DNA Interaction Studies   |   DNA Manipulation
 
Fluorescent Labeling   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
N6-(6-Amino)hexyl-dATP, S pack NU-835S 30 Units 107,37    Add this product to your notepad  
N6-(6-Amino)hexyl-dATP, L pack NU-835L 150 Units 314,50    Add this product to your notepad  
5-Propargylamino-dCTP, S pack NU-809S 30 Units 107,37    Add this product to your notepad  
5-Propargylamino-dCTP, L pack NU-809L 150 Units 314,50    Add this product to your notepad  
Aminoallyl-dUTP, S pack NU-803S 50 Units 107,37    Add this product to your notepad  
Aminoallyl-dUTP, L pack NU-803L 250 Units 314,50    Add this product to your notepad  

 Dye labeled Aminohexyl-dATP

 Dye labeled Propargylamino-dCTP

 Dye labeled Aminoallyl-dUTP

Description
Fluorescent labeling e.g. for FISH or single molecule detection
Incorporation by
DNA Polymerase I
Taq Polymerase, VentR exo- (5-Propargylamino-dCTP and Aminoallyl-dUTP only)

References
Brakmann (2004) Optimal Enzymes for Single-Molecule Sequencing Current Pharmaceutical Biotechnology 5:119.
Tasara et al. (2003) Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA Nucl. Acids Res. 31:2636.
Giller et al. (2003) Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2'-deoxyribonucleoside-5'-triphosphates. Nucl. Acids Res. 31:2630.
 

Genotyping   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads

5'-amino-5'-deoxy nucleotides (e.g. 5'-NH2-dTTP)


Description
Modified nucleotides for DNA sequencing or SNP detection by MALDI-TOF
Please contact nucleotides@jenabioscience.com


Incorporation
Klenow Fragment of DNA Polymerase I
Sequenase v.2.0

References
Wolfe et al. (2002) Synthesis and polymerase incorporation of 5'-amino-2',5'-dideoxy-5'-N-triphosphate nucleotides. Nucl. Acids Res. 30:3739.
Shchepinov et al. (2001) Matrix-induced fragmentation of P3'-N5' phosphoramidate-containing DNA: high-throughput MALDI-TOF analysis of genomic sequence polymorphisms. Nucl. Acids Res. 29:3864.
 

Phasing for x-ray Crystallograpy   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
5Br-dUTP (5-BrdUTP), S pack NU-122S 50 Units 112,74    Add this product to your notepad  
5Br-dUTP (5-BrdUTP), L pack NU-122L 250 Units 330,23    Add this product to your notepad  
5I-dUTP, S pack NU-120S 50 Units 112,74    Add this product to your notepad  
5I-dUTP, L pack NU-120L 250 Units 330,23    Add this product to your notepad  
5Br-dCTP, S pack NU-132S 50 Units 107,37    Add this product to your notepad  
5Br-dCTP, L pack NU-132L 250 Units 314,50    Add this product to your notepad  
5I-dCTP, S pack NU-128S 50 Units 107,37    Add this product to your notepad  
5I-dCTP, L pack NU-128L 250 Units 314,50    Add this product to your notepad  

Description
Incorporation of heavy atom markers into DNA
Incorporation
T7 RNA Polymerase
SP6 RNA Polymerase

References
Holbrook et al. (2001) Crystallization of RNA. Cell. Mol. Life Sci. 58:234.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com