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RNA LabelingRNA Labeling Kits
Jena Bioscience RNA Labeling Kits allow fluorescent labeling of RNA probes in less than two hours.
Thoroughly selected fluorophores are linked to UTP resulting in fluorescent nucleotides with superior properties such as high stability, high signal to noise ratios and high quantum yields. Our RNA Labeling Kits ensure efficient incorporation using T7 in vitro transcription. They are optimized for transcription efficiency and a high degree of labeling.
Typical RNA yields and degrees of labeling (DOL) are given in the table below. Experiments were performed using 500 ng template DNA (523 bp λ-DNA fragment containing a T7 promoter site) in a 20 µl reaction assay.
Thoroughly selected fluorophores are linked to UTP resulting in fluorescent nucleotides with superior properties such as high stability, high signal to noise ratios and high quantum yields. Our RNA Labeling Kits ensure efficient incorporation using T7 in vitro transcription. They are optimized for transcription efficiency and a high degree of labeling.
Typical RNA yields and degrees of labeling (DOL) are given in the table below. Experiments were performed using 500 ng template DNA (523 bp λ-DNA fragment containing a T7 promoter site) in a 20 µl reaction assay.
| Emission Color | Fluorophore | Ex/Em [nm] | Yield | DOL |
| green | Atto 488 | 501/523 | 2 µg | 16.5 |
| yellow-green | Atto 532 | 532/553 | 3 µg | 3.3 |
| yellow | Atto 550 | 554/576 | 1.5 µg | 2.9 |
| red | Atto 647N | 644/669 | 2 µg | 1.5 |
| far-red | Atto 680 | 680/700 | 2.5 µg | 2.2 |
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| Atto 488 RNA Labeling Kit Fluorescent labeling of RNA probes |
FP-220-488 | 10 reactions | 428,00 | 
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| Atto 532 RNA Labeling Kit Fluorescent labeling of RNA probes |
FP-220-532 | 10 reactions | 428,00 |
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| Atto 550 RNA Labeling Kit Fluorescent labeling of RNA probes |
FP-220-550 | 10 reactions | 428,00 |
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| Atto 647N RNA Labeling Kit Fluorescent labeling of RNA probes |
FP-220-647N | 10 reactions | 428,00 |
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| Atto 680 RNA Labeling Kit Fluorescent labeling of RNA probes |
FP-220-680 | 10 reactions | 428,00 |
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Selected References
[1] Uri et al. (2012) Bisubstrate fluorescent probes and biosensors in binding assays for HTS of protein kinase inhibitors. Biochimica et Biophysica Acta 1804:541.
[2] Vaasa et al. (2009) High-affinity bisubstrate probe for fluorescence anisotropy binding/displacement assays with protein kinases PKA and ROCK. Analytical Biochemistry 385(1):85.
[3] Enkvist et al. (2012) Protein-Induced Long Lifetime Luminescence of Nonmetal Probes. ACS chemical biology 6:1052.
[2] Vaasa et al. (2009) High-affinity bisubstrate probe for fluorescence anisotropy binding/displacement assays with protein kinases PKA and ROCK. Analytical Biochemistry 385(1):85.
[3] Enkvist et al. (2012) Protein-Induced Long Lifetime Luminescence of Nonmetal Probes. ACS chemical biology 6:1052.
Please contact info@jenabioscience.com with questions or inquiries.