The JBScreen Membrane Screens 1 - 3 cover 72 of the most successful buffers for crystallization of membrane proteins. Their compositions result from analyzing the crystallization conditions of all membrane proteins crystallized so far.

The JBScreen Membrane crystallization buffers are principally ordered by type and concentration of the precipitant. Like in case of the "classic" JBScreen system, this allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient.

Each kit contains 24 sterile solutions, 10 ml each. All reagents are also available in a pre-filled deep well block.

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.

   Description how the JBScreen buffers are prepared

• Jacobs et al. (2012) Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris. Acta Cryst. F 68:1255.
• Li et al. (2011) Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Crystal Growth & Design 11(2):530.
• Shaw Stewart et al. (2011) Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization. Crystal Growth & Design 11(8):3432.
• Cherezov et al. (2006) In Meso Structure of the Cobalamin Transporter, BtuB, at 1.95 Å Resolution. J. Mol. Biol. 364:716.

Please contact xtals@jenabioscience.com with questions or inquiries.