Catalog JBScreen Family - Pi-minimal ScreenJBScreen Family - Pi-minimal Screen
 
 
JBScreen Classic   |   JBScreen Basic   |   JBScreen Membrane   |   JBScreen Kinase   |   JBScreen Nuc-Pro   |   JBScreen PEG/Salt   |   JBScreen Pentaerythritol   |   JBScreen Cryo   |   JBScreen PACT++   |   JBScreen JCSG++   |   Pi-minimal Screen   |   Pi-PEG Screen   |   JBScreen Wizard
 
Pi minimal Screen
Application

Efficient crystallization screening of soluble proteins

Format

Bulk 96 screening solutions in 10 ml aliquots
HTS 96 screening solutions delivered in a deep-well block, 1.7 ml per well
 
The Pi-minimal Screen was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) for efficient crystallization screening of soluble proteins [1]. The approach is based on incomplete factorial design.

The unique formulation was generated following a strategy named Pi sampling [1] in order to create novel combinations of precipitants, buffers and additives across a standard 96-condition plate layout. Thus, the diversity amongst the crystallization conditions is ideal for initial screening.

The Pi-minimal Screen includes 36 components, i.e. 12 precipitants, 12 buffers systems and 12 salts.
Buffers employed in the Pi-minimal screen are buffer systems (acid-base pairs, e.g. HEPES and HEPES sodium salt). Consequently, pH can be adjusted by mixing 2 stock solutions at different ratios during later optimizations.

The efficiency of the Pi-minimal Screen was demonstrated by the crystallization of 10 proteins before its commercialization [1].

Please make sure you also check out the Pi-PEG Screen!
 
 


Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
 
 
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
Pi-minimal Screen CS-127 4x 24 solutions
(10 ml each)
450,00    Add this product to your notepad      
Pi-minimal Screen HTS CS-211L 96 solutions
(1,7 ml each)
225,00    Add this product to your notepad      

Reference

[1] Gorrec et al. (2011) Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening. Acta Cryst. D67:463.

Available online at http://journals.iucr.org/d/issues/2011/05/00/bw5391/index.html
 


Please contact xtals@jenabioscience.com with questions or inquiries.