Catalog DBCO Reagents - (Desthio) Biotinylation ReagentsDBCO Reagents - (Desthio) Biotinylation Reagents
 
 
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Labeling of a molecule (e.g. a protein or nucleic acid) with biotin is routinely performed for its subsequent affinity purification via streptavidin agarose or detection with fluorescent or HRP-labeled streptavidin. Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions. Desthiobiotin however, binds less tightly to streptavidin (KD = 10-11 M) and desthiobiotinylated molecules are therefore easily eluted from the complex in the presence of excess Biotin.

Dibenzocyclooctyne (DBCO)*-containing (desthio) biotinylation reagents can be used for the labeling of Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).

A number of (desthio) biotinylation reagents with

  • different solubility characteristics (PEG- and Sulfo-linker for increased molecule solubility)
  • different cleavability characteristics (chemically or photocleavable linke)
are available to fit specific application & molecule requirements.

*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)

Click Chemistry background information
 


Table 1: Available DBCO-containing (Desthio) Biotinylation Reagents

LabelDBCO
BiotinDBCO-PEG4-Biotin
Sulfo-DBCO-Biotin
Biotin with chemically cleavable linkerDiazo Biotin-DBCO
Dde Biotin-DBCO
Biotin with photocleavable linkerPhotocleavable Biotin-DBCO
Photocleavable Biotin-Sulfo-DBCO
DesthiobiotinDBCO-PEG4-Desthiobiotin
 

DBCO-PEG4-Biotin Conjugate
Dibenzylcyclooctyne-PEG4-Biotin Conjugate
   
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-A105P4-10 10 mg 152,21    Add this product to your notepad  
100 mg pack CLK-A105P4-100 100 mg 917,38    Add this product to your notepad

 
Sulfo-DBCO-Biotin Conjugate
Sulfo-Dibenzylcyclooctyne-Biotin Conjugate
   
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-A116-10 10 mg 152,21    Add this product to your notepad  
100 mg pack CLK-A116-100 100 mg 1.259,13    Add this product to your notepad

 
Diazo Biotin-DBCO    
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-1043-10 10 mg 188,07    Add this product to your notepad  
100 mg pack CLK-1043-100 100 mg 1.150,44    Add this product to your notepad

 
Dde Biotin-DBCO    
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-1138-10 10 mg 167,70    Add this product to your notepad  
100 mg pack CLK-1138-100 100 mg 1.163,50    Add this product to your notepad

 
DBCO-PEG4-Desthiobiotin
Dibenzylcyclooctyne-PEG4-Desthiobiotin
   
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-1108-10 10 mg 193,70    Add this product to your notepad  
100 mg pack CLK-1108-100 100 mg 1.163,50    Add this product to your notepad

 
Photocleavable Biotin-DBCO    
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-1120-10 10 mg 193,70    Add this product to your notepad  
100 mg pack CLK-1120-100 100 mg 1.163,50    Add this product to your notepad

 
Photocleavable Biotin-Sulfo-DBCO    
Product Cat. No. Amount Price (EUR) Buy / Note  
10 mg pack CLK-1121-10 10 mg 193,70    Add this product to your notepad  
100 mg pack CLK-1121-100 100 mg 1.163,50    Add this product to your notepad

 
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