Catalog Halogenated, Mercurated and Selenium-containing Nucleotides and Oligonucleotides - Halogenated Adenosine NucleotidesHalogenated, Mercurated and Selenium-containing Nucleotides and Oligonucleotides - Halogenated Adenosine Nucleotides
 
 
Halogenated Adenosine Nucleotides   |   Halogenated Guanosine Nucleotides   |   Other Halogenated Nucleotides   |   Mercurated Nucleotides   |   Selenium-containing Nucleotides
 

Halogenated nucleotides as rational phasing tools for protein crystallography

The most simple method currently used for solution of the macromolecular phase problem, single wavelength anomalous dispersion (SAD), still involves the incorporation of heavy atoms into protein crystals. After crystallization, finding such derivatives is the second major bottleneck in the determination of the 3D structure of bio-macromolecules.

Most labelling procedures focus on the protein itself in a "trial and error" fashion. Halogenated ATP and GTP analogs however, provide an alternative method that allows rational incorporation of heavy atoms into a large number of physiologically relevant enzymes:
  • In the human genome alone, estimated 5,000 to 10,000 proteins interact with ATP or GTP (protein- and nucleotide-kinases, motor proteins, chaperones... or the superfamily of GTPases, respectively).
  • The incorporation of iodine or bromine allows experimental phasing for proteins with molecular weights of at least up to 50 kDa. Importantly, for MIR and MIRAS experiments, such derivatives are likely to be isomorphous with the native crystal.
  • The kinetics of binding of 2'-halogenated ATP analogs to most enzymes so far investigated (including myosin, creatine kinase, hexokinase, pyruvate kinase and UMP/CMP kinase) are similar to those of non-substituted ATP.
  • The complexes of 2'-halogenated GTP analogs with the GTPases p21ras or Rab5 have dissociation constants well suitable for crystallization.
  • Several proteins including kinesin, non-claret disjunctional protein and TMP kinase have been crystallized with 2'-halogenated analogs.
 
Also check out our JBS Halo Kits, containing a selection of important Halo-ATP and Halo-GTP analogs!
 

2'-Iodo-dAMP
(2'I-dAMP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
pack NU-147 250 Units 322,36    Add this product to your notepad  

 
2'-Iodo-dADP
(2'I-dADP)
   
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pack NU-109 150 Units 322,36    Add this product to your notepad  

 
2'-Iodo-dATP
(2'I-dATP)
   
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pack NU-101 150 Units 338,49    Add this product to your notepad  

 
2'-Iodo-dAppNHp
(2'I-dAppNHp, 2'I-dAMPPNP)
   
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pack NU-103 100 Units 322,36    Add this product to your notepad  

 
2'-Bromo-dADP
(2'Br-dADP)
   
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pack NU-110 150 Units 322,36    Add this product to your notepad  

 
2'-Bromo-dATP
(2'Br-dATP)
   
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pack NU-102 150 Units 322,36    Add this product to your notepad  

 
2'-Bromo-dAppNHp
(2'Br-dAppNHp, 2'Br-dAMPPNP)
   
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pack NU-104 100 Units 322,36    Add this product to your notepad  

 
2'-Bromo-dAP2(Nic)
(2'Br-dAP2(Nic))
   
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pack NU-524 300 Units 1.017,31    Add this product to your notepad  

 
8-Iodo-ADP
(8I-ADP)
   
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S pack NU-111S 50 Units 110,05    Add this product to your notepad  
L pack NU-111L 5 x 50 Units 322,36    Add this product to your notepad

 
8-Iodo-ATP
(8I-ATP)
   
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S pack NU-113S 50 Units 110,05    Add this product to your notepad  
L pack NU-113L 5 x 50 Units 322,36    Add this product to your notepad

 
8-Iodo-AppNHp
(8I-AppNHp, 8I-AMPPNP)
   
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pack NU-115 100 Units 322,36    Add this product to your notepad  

 
8-Bromo-cAMP
(8Br-cAMP)
   
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S pack NU-1502S 10 mg 110,05    Add this product to your notepad  
L pack NU-1502L 50 mg 322,36    Add this product to your notepad

 
8-Bromo-ADP
(8Br-ADP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-112S 100 Units 110,05    Add this product to your notepad  
L pack NU-112L 5 x 100 Units 322,36    Add this product to your notepad

 
8-Bromo-ATP
(8Br-ATP)
   
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S pack NU-114S 100 Units 110,05    Add this product to your notepad  
L pack NU-114L 5 x 100 Units 322,36    Add this product to your notepad

 
8-Bromo-AppNHp
(8Br-AppNHp, 8Br-AMPPNP)
   
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pack NU-116 100 Units 322,36    Add this product to your notepad  

 
8-Bromo-dATP
(8Br-dATP)
   
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S pack NU-117S 50 Units 110,05    Add this product to your notepad  
L pack NU-117L 5 x 50 Units 322,36    Add this product to your notepad

 
8-Bromo-AP2(Nic)
(8Br-AP2(Nic))
   
Product Cat. No. Amount Price (EUR) Buy / Note  
pack NU-523 300 Units 1.017,31    Add this product to your notepad  

 
8-Bromo-2',5'-pAp
(8Br-2',5'-pAp)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-136S 30 Units 110,05    Add this product to your notepad  
L pack NU-136L 5 x 30 Units 322,36    Add this product to your notepad

 
8-Bromo-3',5'-pAp
(8Br-3',5'-pAp)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-137S 30 Units 110,05    Add this product to your notepad  
L pack NU-137L 5 x 30 Units 322,36    Add this product to your notepad

 
7-Deaza-7-Iodo-dATP
(7-Deaza-7-I-dATP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-1181S 50 Units 110,05    Add this product to your notepad  
L pack NU-1181L 5 x 50 Units 322,36    Add this product to your notepad

 
2'-Chloro-dATP
(2'Cl-dATP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-148S 50 Units 110,05    Add this product to your notepad  
L pack NU-148L 5 x 50 Units 322,36    Add this product to your notepad

 
7-Deaza-7-Bromo-dATP
(7-Deaza-7-Br-dATP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-149S 50 Units 110,05    Add this product to your notepad  
L pack NU-149L 5 x 50 Units 322,36    Add this product to your notepad

 
2'-Fluoro-dATP
(2'F-dATP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-151S 50 Ál
(100 mM)
85,00    Add this product to your notepad  
L pack NU-151L 5 x 50 Ál
(100 mM)
340,00    Add this product to your notepad

 
2'-Fluoro-dAMP
(2'F-dAMP)
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack NU-152S 50 Units 110,05    Add this product to your notepad  
L pack NU-152L 5 x 50 Units 322,36    Add this product to your notepad

 

Selected References

  • Gruen et al. (1999) 2'-Halo-ATP and -GTP analogues: Rational phasing tools for protein crystallography. Protein Sci. 8:2524.
  • Gruen et al. (1999) Synthesis of 2'-iodo- and 2'-bromo-ATP and GTP analogues as potential phasing tools for X-ray crystallography. Nucleos. Nucleot. 18 (1):137.
  • Naber et al. (1995) A novel adenosine-triphosphate analog with a heavy-atom to target the nucleotide-binding site of proteins. Protein Sci. 4:1824.
 
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