The ability to purify recombinant proteins using affinity chromatography has greatly advanced protein research.

In affinity chromatography, the protein of interest is purified by its ability to bind a specific ligand that is immobilized on a chromatographic bead material (matrix). This matrix is usually packed into a column.

Crude cell-lysates are loaded onto the column under conditions that ensure specific binding of the protein to the immobilized ligand. Other proteins that do not bind the immobilized ligand are washed through. Elution of the bound protein of interest can be achieved by changing the experimental conditions to favour desorption.

Jena Bioscience offers a wide range of affinity chromatography products for different applications. Click on the links in the image below to visit the appropriate product sections:

 
Protein G AgaroseImmobilized O-Phospho-TyrosineImmobilized O-Phospho-ThreonineImmobilized Inosine NucleotidesImmobilized Thymidine NucleotidesImmobilized Cytidine NucleotidesAffinity Chromatography Kits for ATP binding ProteinsImmobilized Xanthosine NucleotidesImmobilized Uridine NucleotidesImmobilized Guanosine/m7-Guanosine NucleotidesGST-Cleavage Capture KitImmobilized O-Phospho-SerineImmobilized Adenosine NucleotidesProtein A AgaroseGlutathione Agarose, Fast FlowPhospho-Aminoacid binding ProteinsNucleotide binding ProteinsAntibody ImmobilizationHis-tagged ProteinsGST-tagged ProteinsNickel, Zinc, Cobalt, Copper and Metal Free Agaroses, Gravity FlowNickel and Cobalt Agaroses, Fast FlowNickel NTA Agarose, Fast FlowAffinity Chromatography - Main Navigation
 
Also available: Auxiliary Equipment, such as empty Columns, Spin Columns or Cartridges.
 
Affinity Chromatography Mini Catalog

  PDF download Download the Affinity Chromatography Mini Catalog in PDF format (2.9 MB)