Cell Penetrating Peptides

The internalization of bioactive cargo into live cells has become an area of fast growth. In particular, the use of Cell Penetrating Peptides (CPPs) as helper-molecules for overcoming cell membrane barriers enabled researchers to transfect numerous cell lines and cell types with a large number of proteins & enzymes, nucleotides, and nucleic acids.

In our CPP section we offer novel kits and formulations that
  • are applicable to many cell lines including both adherent and suspension cells
  • have low toxicity for live cells
  • are targeted to different internalization mechanisms thereby achieving higher success rates
  • enable efficient transduction of nearly 100%
  • minimize artefacts due to complete post-internalizational break down of CPPs
  • allow straight forward experiments, no expensive equipment necessary
  • contain selected helper reagents for fine-tuning of experimental set ups
  • are safe to use without any special precautions

For detailed information please refer to the   General Manual.

 

Optimized Protein & Nucleotide Internalization Mixes

Optimized CPP-cocktails for the efficienct internalization of proteins and nucleotides into your cell line of interest
 

Selected Single Cell Penetrating Peptides (CPPs)

Selected single cell penetrating peptides (CPPs) for protein and nucleotide internalization
 

Fluorescent Cargo

Fluorescent BSA and nucleotides as internalization positive control
 

Auxiliary Compounds

Streamlined selection of reagents for the optimization of internalisation experiments
 

Selected References

  • Mussbach et al. (2011) Transduction of Peptides and Proteins Into Live Cells by Cell Penetrating Peptides. Journal of Cellular Biochemistry 112:3824
 
  • Handbook of Cell-Penetrating Peptides, Second Edition, Ed. by . Langel, CRC Taylor and Francis, Boca Raton, London, New York (2007).
  • Meade et al. (2008) Enhancing the cellular uptake of siRNA duplexes following non-covalent packaging with protein transduction domain peptides. Adv. Drug Deliv. Rev. 60:530.
  • Prochiantz (2005) Protein transduction: from physiology to technology and vice versa. Adv. Drug Deliv. Rev. 57:491.
    Me et al. (2006) Cell-penetrating peptides as vectors for peptide, protein and oligonucleotide delivery. Curr. Opin. Pharmacol. 6:509.
  • Deshayes et al. (2008) Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy. Adv. Drug Deliv. Rev. 60:537.