Crystal cryoprotection has become a fundamental method for studying biological macromolecules. In many cases cryoprotection is essential for obtaining a complete data set by overcoming the problem of radiation damage. Moreover, cryomethods allow crystallographers to work with smaller crystals, and cryocooled crystals can be stored indefinitely without losing diffraction quality.

Cryoprotectants provide a means to protect macromolecular crystals from the damaging effects of ice formation during the cryocooling process.

JBScreen Cryo is an advanced screen for cryo conditions. The four JBScreen Cryo kits contain the 96 most successful crystallization conditions proven to be suitable for cryo crystallography. Just like in the case of the JBScreen Classic, the formulations of JBScreen Cryo were determined by extensive data mining.

JBScreen Cryo is available in the standard 10 ml bulk format or in pre-filled 96-well deep well plates - JBScreen Cryo HTS.

JBScreen Cryo can be used for standard vapor diffusion setups to determine conditions suitable for cryo crystallography "from scratch" or to find a suitable cryo condition when crystals were grown with a standard (non-cryo) screen.

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.

   More information: JBScreen Cryo User Guide

   Description how the JBScreen buffers are prepared

Fislage et al. (2011) Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus. Acta Cryst. F 67(2):1432.

Please contact xtals@jenabioscience.com with questions or inquiries.