Catalog JBScreen Family - JBScreen MembraneJBScreen Family - JBScreen Membrane
 
 
JBScreen Classic   |   JBScreen Basic   |   JBScreen Membrane   |   JBScreen Kinase   |   JBScreen Nuc-Pro   |   JBScreen PEG/Salt   |   JBScreen Pentaerythritol   |   JBScreen PACT++   |   JBScreen JCSG++   |   Pi-minimal Screen   |   Pi-PEG Screen   |   JBScreen Wizard
 
JBScreen bulk & HTS
The JBScreen Membrane Screens 1 - 3 cover 72 of the most successful buffers for crystallization of membrane proteins. Their compositions result from analyzing the crystallization conditions of all membrane proteins crystallized so far.

The JBScreen Membrane crystallization buffers are principally ordered by type and concentration of the precipitant. Like in case of the "classic" JBScreen system, this allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient.

Each kit contains 24 sterile solutions, 10 ml each. All reagents are also available in a pre-filled deep well block.

 
Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
 
 
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
JBScreen Membrane 1
(PEG 400 to PEG 2000 MME based)
CS-301L 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Membrane 2
(PEG 2000 MME to PEG 10000 based)
CS-302L 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Membrane 3
(Ammonium Sulfate, Alcohol and Salt based)
CS-303L 24 solutions
(10 ml each)
150,00    Add this product to your notepad      
JBScreen Membrane HTS CS-305L 72 solutions
(1,7 ml each)
170,00    Add this product to your notepad      
JBScreen Membrane 1 - 3 CS-306L 3 Kits 340,00    Add this product to your notepad
JBScreen Membrane 1 - 3 & JBScreen Detergents CS-307L 3 + 1 Kits 470,00    Add this product to your notepad

Selected Literature Citations of JBScreen Membrane

  • Jacobs et al. (2012) Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris. Acta Cryst. F 68:1255.
  • Li et al. (2011) Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Crystal Growth & Design 11(2):530.
  • Shaw Stewart et al. (2011) Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization. Crystal Growth & Design 11(8):3432.
  • Caffrey et al. (2009) Crystallizing Membrane Proteins Using Lipidic Mesophases. Nat Protoc. 4:706.
  • Cherezov et al. (2006) In Meso Structure of the Cobalamin Transporter, BtuB, at 1.95 Resolution. J. Mol. Biol. 364:716.
 
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