Catalog LEXSY Configurations - Inducible (Episomal)LEXSY Configurations - Inducible (Episomal)
 
 
Inducible (Genome integrated)   |   Inducible (Episomal)   |   Constitutive (Genome integrated)   |   Vectors and Primers   |   In Vitro (cell-free)   |   Custom Expression
 

The episomal inducible 4th generation of LEXSY makes use of amplification and oligomerisation of expression plasmids maintained extrachromosomally as self-replicating episomes in the LEXSY host cells, providing enhanced number of target gene copies for transcription by T7 RNA polymerase.


It was shown for a number of proteins, that the episomal inducible expression achieved 5 - 10-fold higher yields than integrative inducible expression. High expression strains are obtained by clonal selection and screening which is based on fluorescence intensity resulting from the co-expressed BleCherry gene. Primary screening on solid media can be performed in daylight since the Cherry colour is visible by eye.

 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
LEXSinduce4 Expression Kit
contains the episomal vector pLEXSY_IE-blecherry4
for inducible cytosolic or secretory protein expression
EGE-1420blecherry 1 Kit 2.250,00    Add this product to your notepad  

Each kit contains:
  • pLEXSY_IE-blecherry4 expression vector
  • LEXSY host T7-TR as glycerol stocks
  • all components for preparation of 1 L LEXSY BHI medium
  • all selective antibiotics for 1 L medium
  • primer sets for insert sequencing and diagnostic PCR
  • an easy-to-follow manual, see PDF file in "Downloads"

All components of the kit are available also separately.
Download the LEXSY vector map and sequence at:
 
We also provide all the molecular tools required for the construction of expression plasmids, such as


Please contact expression@jenabioscience.com with questions or inquiries.