The episomal inducible 4th generation of LEXSY makes use of amplification and oligomerisation of expression plasmids maintained extrachromosomally as self-replicating episomes in the LEXSY host cells, providing enhanced number of target gene copies for transcription by T7 RNA polymerase.

It was shown for a number of proteins, that the episomal inducible expression achieved 5 - 10-fold higher yields than integrative inducible expression. High expression strains are obtained by clonal selection and screening which is based on fluorescence intensity resulting from the co-expressed BleCherry gene. Primary screening on solid media can be performed in daylight since the Cherry colour is visible by eye.

   View reference publication here

Each kit contains:

• pLEXSY_IE-blecherry4 expression vector
• LEXSY host T7-TR as glycerol stocks
• all components for preparation of 1 L LEXSY BHI medium
• all selective antibiotics for 1 L medium
• primer sets for insert sequencing and diagnostic PCR
• an easy-to-follow manual, see PDF file in "Downloads"

All components of the kit are available also separately.
Download the LEXSY vector map and sequence at:

• List of all LEXSY Vectors

We also provide all the molecular tools required for the construction of expression plasmids, such as

• PCR tools, DNA isolation, purification and sequencing tools
• restriction enzymes, ligases

Please contact expression@jenabioscience.com with questions or inquiries.