. . Core Kits, Standard PCR, Molecular Biology, Products & Ordering, Jena Bioscience
 
Catalog Standard PCR - Core KitsStandard PCR - Core Kits
 
 
Direct and Multiplex PCR   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   dNTP Mixes   |   dNTP Bundles and Single Solutions   |   Supplements   |   School and Demo Kits
 
Standard PCR Core Kits
Complete sets of reagents required for PCR

Core Kits consist of all reagents required for PCR (except template and primer). Components (polymerase, dNTP-Mix, complete reaction buffer containing MgCl2) are provided individually. Additional MgCl2 stock solution allows optimization of magnesium-sensitive PCR reactions. Depending on focus of particular application (yield, specificity, accuracy) various types of enzymes are offered.

Standard PCR - Product Selection Guide
 

Taq Core Kit
Kit of thermostable DNA polymerase, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-232S 200 units 48,00    Add this product to your notepad  
L pack PCR-232L 1000 units 192,00    Add this product to your notepad

 
Taq Core Kit - red dyed
Kit of thermostable DNA polymerase, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-232S-red 200 units 48,00    Add this product to your notepad  
L pack PCR-232L-red 1000 units 192,00    Add this product to your notepad

 
Taq Core Kit / high yield
Kit of thermostable DNA polymerase, dNTPs and high yield buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-231S 200 units 48,00    Add this product to your notepad  
L pack PCR-231L 1000 units 192,00    Add this product to your notepad

 
Taq Core Kit / high yield - red dyed
Kit of thermostable DNA polymerase, dNTPs and high yield buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-231S-red 200 units 48,00    Add this product to your notepad  
L pack PCR-231L-red 1000 units 192,00    Add this product to your notepad

 
Hot Start Core Kit
Kit of heat-activatable DNA polymerase for high specificity, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-233S 200 units 83,00    Add this product to your notepad  
L pack PCR-233L 1000 units 332,00    Add this product to your notepad

 
Hot Start Core Kit - red dyed
Kit of heat-activatable DNA polymerase for high specificity, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-233S-red 200 units 83,00    Add this product to your notepad  
L pack PCR-233L-red 1000 units 332,00    Add this product to your notepad

 
High Fidelity Core Kit
Kit of thermostable DNA polymerase for high accuracy, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-234S 100 units 56,00    Add this product to your notepad  
L pack PCR-234L 500 units 224,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Pol (2.5 units/l)
dNTP Mix
High Fidelity Buffer - 10 x conc.
High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.
 
High Fidelity Hot Start Core Kit
Kit of heat-activatable DNA polymerase for high accuracy and specificity, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-235S 100 units 76,00    Add this product to your notepad  
L pack PCR-235L 500 units 304,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

High Fidelity Hot Start Pol (2.5 units/l)
dNTP Mix
High Fidelity Buffer - 10 x conc.
High Fidelity Hot Start Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.
 
Pfu-X Core Kit
Kit of proofreading DNA polymerase for highest accuracy, dNTPs and reaction buffer
   
Product Cat. No. Amount Price (EUR) Buy / Note  
S pack PCR-237S 100 units 68,00    Add this product to your notepad  
L pack PCR-237L 500 units 272,00    Add this product to your notepad

Store at -20C, avoid frequent thawing and freezing

Pfu-X Polymerase (2.5 units/l)
dNTP Mix
Pfu-X Buffer - 10 x conc.
Pfu-X Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.
 

For inquiries or further information, please contact us at pcr@jenabioscience.com