Mechanisms affecting gene regulation attained increased significance over the past years and created the rapidly expanding field of Epigenetics. Since control of transcription affects cell differentiation, development and homeostasis, severe diseases such as cancer are correlated with epigenetic disorders.

Role of NCoR and SMRT in Epigenetics
Nuclear receptors play a pivotal role in gene activation and repression. By binding to specific promoter elements they recruit a variety of cofactors including the corepressors NCoR and SMRT that interact with unliganded nuclear receptors through the so-called CoRNR box. These cofactors are essential for recruiting additional effector proteins (such as HDACs), thereby forming a functional holocomplex that leads to gene silencing. [1-3]

Application of our antibodies
Our NCoR and SMRT antibodies are tested for Western Blot (WB) and Immunoprecipitation (IP). Anti-NCoR (ABD-039) can be further applied for Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry. [4]

[1] Rosenfeld et al. (2006) Sensors and signals: a coactivator / corepressor / epigenetic code for integrating signal-dependent programs of transcriptional response. Genes Dev. 20:1405.

[2] Heinzel et al. (1997) A complex containing N-CoR, mSln3 and histone deacetylase mediates transcriptional repression. Nature 387:43.

[3] Nagy et al. (1997) Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase. Cell 89:373.

[4] Tiefenbach et al. (2006) SUMOylation of the Corepressor N-CoR Modulates Its Capacity to Repress Transcription. Mol. Biol. Cell. 17:1643.

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